Use of Comparative Transcriptomics Combined With Physiological Analyses to Identify Key Factors Underlying Cadmium Accumulation in Brassica juncea L.
Use of Comparative Transcriptomics Combined With Physiological Analyses to Identify Key Factors Underlying Cadmium Accumulation in Brassica juncea L.
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DOI：
10.3389/fgene.2021.655885
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发表时间：
2021
影响因子：
3.7
通讯作者：
Yan M
中科院分区：
文献类型：
--
作者：
Zhang D;Du Y;He D;Zhou D;Wu J;Peng J;Liu L;Liu Z;Yan M
土壤镉（Cd）污染已成为一个需要解决的严重环境问题。阐明镉积累的机制可能有助于同时积累高量和低量镉的植物的发育。在本研究中，结合表型、生理和比较转录组分析，研究了不同 Cd 浓度（0、5、10、30、50 mg/kg）对芥菜的影响。我们的结果表明，芥菜幼苗对 5 mg/kg Cd 处理具有一定程度的耐受性，而较高的 Cd 胁迫（10-50 mg/kg）可以抑制芥菜的生长。 芥菜L.幼苗。 30、50 mg/kg Cd处理芥菜可溶性蛋白、丙二醛含量升高，但过氧化氢酶活性、可溶性糖和叶绿素含量降低。比较转录组分析表明，XTH18（木葡聚糖内转葡糖基酶/水解酶）、XTH22 和 XTH23 下调，但 PME17（果胶甲酯酶）和 PME14 上调，这可能有助于细胞壁完整性的维持。此外，HMA3（重金属ATP酶3）的下调和Nramp3（自然抗性相关巨噬细胞蛋白3）、HMA2（重金属ATP酶2）和Nramp1（自然抗性相关巨噬细胞蛋白1）的上调也可能在降低根部镉毒性方面发挥作用。综上所述，我们的研究结果可能有助于阐明芥菜对不同浓度镉的反应机制。
The contamination of soils with cadmium (Cd) has become a serious environmental issue that needs to be addressed. Elucidating the mechanisms underlying Cd accumulation may facilitate the development of plants that accumulate both high and low amounts of Cd. In this study, a combination of phenotypic, physiological, and comparative transcriptomic analyses was performed to investigate the effects of different Cd concentrations (0, 5, 10, 30, 50 mg/kg) on Brassica juncea L. Our results suggest that B. juncea L. seedlings had a degree of tolerance to the 5 mg/kg Cd treatment, whereas higher Cd stress (10–50 mg/kg) could suppress the growth of B. juncea L. seedlings. The contents of soluble protein, as well as MDA (malondialdehyde), were increased, but the activities of CAT (catalase) enzymes and the contents of soluble sugar and chlorophyll were decreased, when B. juncea L. was under 30 and 50 mg/kg Cd treatment. Comparative transcriptomic analysis indicated that XTH18 (xyloglucan endotransglucosylase/hydrolase enzymes), XTH22, and XTH23 were down-regulated, but PME17 (pectin methylesterases) and PME14 were up-regulated, which might contribute to cell wall integrity maintenance. Moreover, the down-regulation of HMA3 (heavy metal ATPase 3) and up-regulation of Nramp3 (natural resistance associated macrophage proteins 3), HMA2 (heavy metal ATPase 2), and Nramp1 (natural resistance associated macrophage proteins 1) might also play roles in reducing Cd toxicity in roots. Taken together, the results of our study may help to elucidate the mechanisms underlying the response of B. juncea L. to various concentrations of Cd.
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