Estrogen receptor mRNA expression patterns in the liver and ovary of female rainbow trout over a complete reproductive cycle.
Estrogen receptor mRNA expression patterns in the liver and ovary of female rainbow trout over a complete reproductive cycle.
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DOI：
10.1016/j.ygcen.2012.06.010
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发表时间：
2012-09-15
影响因子：
2.7
通讯作者：
Hayton, William L.
中科院分区：
文献类型：
--
作者：
Nagler, James J.;Cavileer, Timothy D.;Verducci, Joseph S.;Schultz, Irvin R.;Hook, Sharon E.;Hayton, William L.
雌激素是生殖过程中的关键激素，需要与靶器官中的雌激素受体结合才能发挥生物活性。鱼类有两种不同的雌激素受体亚型，α(α)和β(β)，每个亚型的额外亚型的不同亚型的不同组合取决于分类单元内基因组复制的历史。雌激素受体异构体在雌性生殖周期中的比较表达模式将为了解每一种受体的独特功能和重要性提供重要的见解。本研究的目的是检测成年雌性虹鲑鱼在一年的生殖周期中肝脏和卵巢中四种雌激素受体亚型(erα1，erα2，erβ1，erβ2)的mRNAs。实时定量RT-PCR检测雌激素受体mRNA亚型的表达。几项生殖指标(性腺指数、最大卵母细胞直径、血浆雌二醇-17β、血浆卵黄蛋白原和排卵量)也被量化以进行比较，并用于相关性分析以检验任何相互关系。在4种亚型中，erα1在肝脏中的表达最高，且与肝脏中erβ1的表达呈显著正相关。肝脏中ER-α-2mRNA的表达水平最低，但与卵巢最大卵母细胞直径呈显著正相关。肝脏中Erβ亚型的表达模式是先升高，然后随着生殖发育的进行而逐渐降低。在卵巢中，ERβ1亚型在所有雌激素受体亚型中的表达最高，在生殖周期开始时，但随后下降。两种卵巢ER-β亚型之间均呈显著正相关。相反，ER-α-2mRNA在卵巢中的表达水平在接近周期结束时达到最高水平，并与血浆雌二醇-17β水平呈显著正相关，性腺指数、最大卵母细胞直径和卵黄蛋白原水平也出现在最高水平。
Estrogens are critical hormones involved in reproduction and need to bind to estrogen receptors in target organs for biological activity. Fishes have two distinct estrogen receptor subtypes, alpha (α) and beta (β), with variable combinations of additional isoforms of each subtype dependent on the history of genome duplication within a taxon. The comparative expression patterns of estrogen receptor isoforms during the female reproductive cycle will provide important insights into the unique function and importance of each. The purpose of this study was to measure the mRNAs for the four estrogen receptor isoforms (erα1, erα2, erβ1, erβ2) in the liver and ovary of adult, female rainbow trout over the course of an annual reproductive cycle. The expression of estrogen receptor mRNA isoforms was measured by quantitative real-time RT-PCR. Several reproductive indices (gonadosomatic index, maximum oocyte diameter, plasma estradiol-17β, plasma vitellogenin, and ovulation) were also quantified for comparison and used in a correlation analysis to examine any inter-relationships. Of the four isoforms, the expression of erα1 was highest in the liver, and had a significant positive correlation with liver erβ1 expression. Liver expression of erα2 mRNA was the lowest, but showed a significant positive correlation with maximum oocyte diameter in the ovary. The pattern of the erβ isoforms in liver was one of initially elevated mRNA expression followed by a gradual decrease as reproductive development proceeded. In the ovary the erβ1 isoform had the highest mRNA expression of all estrogen receptor isoforms, at the beginning of the reproductive cycle, but then decreased afterward. Both ovarian erβ isoforms had a significant positive correlation with one another. In contrast, erα2 mRNA expression showed a high maximum level in the ovary near the end of the cycle along with a significant positive correlation with plasma estradiol-17β levels; the highest gonadosomatic indices, maximum oocyte diameter, and vitellogenin levels occurred then too.
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