Cell manipulation in autologous chondrocyte implantation: from research to cleanroom
Cell manipulation in autologous chondrocyte implantation: from research to cleanroom
复制标题
自体软骨细胞植入中的细胞操作：从研究到洁净室
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
P. Fornasari
中科院分区：
文献类型：
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作者：
L. Roseti;M. Serra;D. Tigani;Irene Brognara;Annamaria Lopriore;A. Bassi;P. Fornasari
在骨科领域，自体软骨细胞植入是目前用于受损关节软骨再生的技术。有证据表明，新形成的组织显示出与透明软骨相似的特征。体外软骨细胞操作是这种治疗性治疗的关键阶段，包括不同的步骤：从软骨活检中分离细胞，单层培养中的扩增和在三维生物材料上生长以植入受损区域。为了最大限度地降低体外细胞污染的风险，操作必须在受控环境（如洁净室）中进行。此外，选择适合人类临床使用的试剂和原材料以及将研究方案转化为标准化生产流程也很重要。在这项研究中，我们描述了软骨细胞操作协议（分离和单层扩增）在洁净室自体移植的应用程序的发展所获得的初步结果。
In the field of orthopaedics, autologous chondrocyte implantation is a technique currently used for the regeneration of damaged articular cartilage. There is evidence of the neo-formation of tissue displaying characteristics similar to hyaline cartilage. In vitro chondrocyte manipulation is a crucial phase of this therapeutic treatment consisting of different steps: cell isolation from a cartilage biopsy, expansion in monolayer culture and growth onto a three-dimensional biomaterial to implant in the damaged area. To minimise the risk of in vitro cell contamination, the manipulation must be performed in a controlled environment such as a cleanroom. Moreover, the choice of reagents and raw material suitable for clinical use in humans and the translation of research protocols into standardised production processes are important. In this study we describe the preliminary results obtained by the development of chondrocyte manipulation protocols (isolation and monolayer expansion) in cleanrooms for the application of autologous implantation.
影响因子：
2.8
作者：
KUETTNER, KE
通讯作者：
KUETTNER, KE
用于研究急性机械损伤对软骨影响的体外模型。
影响因子：
4.2
作者：
Patwari,P;Fay,J;Cook,MN;Badger,AM;Kerin,AJ;Lark,MW;Grodzinsky,AJ
通讯作者：
Grodzinsky,AJ