Enzymatic reduction of 11-cis-retinal bound to cellular retinal-binding protein.
Enzymatic reduction of 11-cis-retinal bound to cellular retinal-binding protein.
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酶促还原 11-顺式视黄醛与细胞视黄醛结合蛋白的结合。
DOI：
10.1016/0304-4165(82)90277-x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Bredberg,L
中科院分区：
文献类型：
--
作者：
Saari,JC;Bredberg,L
牛视网膜细胞视黄醇结合蛋白纯化结合11-顺式-视黄醇和11-顺式-视黄醇作为内源性配体。由于这些类维生素A可通过脱氢酶反应相互转化，因此确定了结合的11-顺式-视黄醇的醛官能团对化学和酶促还原剂的可及性。来自视网膜色素上皮微粒体的11-顺式-视黄醇脱氢酶，首先由Lion，F.，Rotmans，J.P.，Daemen，F.J.M.和Bonting，S.L.（Biochim. Biophys. Acta 384，283-292，1975）被发现在pH 5.5和37°C下快速且几乎定量地还原复合的11-顺式-视黄醛。还原产物11-顺式-视黄醇在反应后仍与结合蛋白复合。还原进行3倍，更快地与NADH比与NADPH。在反应过程中没有发生几何异构构型的变化。在pH 8.5时，在NAD存在下，视网膜色素上皮的脱氢酶氧化与细胞视网膜结合蛋白复合的11-顺式-视黄醇。尽管与细胞视黄醇结合蛋白复合的11-顺式-视黄醛的酶促还原反应已经完成，但醛的功能对于几种化学还原剂来说是不可接近的。将复合物与NaBH 4（pH 7.5）和NaCNBH 3（pH 5.5）或硼烷二甲胺（pH 5.5）一起孵育，除非复合物暴露于白色光，否则不会导致11-顺式-视黄醛的还原，这种处理已知会产生对结合蛋白几乎没有亲和力的全反式-视黄醛。在37°C下，当以产生约60%的未复合类维生素A减少的量加入时，在15分钟内，肝醇脱氢酶仅产生10%的与细胞视黄醇结合蛋白复合的11-顺式-视黄醇减少。结果表明，11-顺式视黄醇脱氢酶与细胞视黄醇结合蛋白复合物之间的相互作用是特异性的，结合蛋白可能作为脱氢酶的底物载体。
Cellular retinal-binding protein from bovine retina purifies with bound 11-cis-retinal and 11-cis-retinol as endogenous ligands. Inasmuch as these retinoids are interconvertible by a dehydrogenase reaction the accessibility of the aldehyde function of bound 11-cis-retinal to chemical and enzymatic reducing agents was determined. An 11-cis-retinol dehydrogenase from retinal pigment epithelial microsomes, first described by Lion, F., Rotmans, J.P., Daemen, F.J.M. and Bonting, S.L. (Biochim. Biophys. Acta 384, 283–292, 1975) was found to reduce complexed 11-cis-retinal at pH 5.5 and 37°C rapidaly and nearly quantitatively. The product of the reduction, 11-cis-retinol, remained complexed with the binding protein following the reaction. Reduction proceeded 3-times more rapidly with NADH than with NADPH. No change in geometrical isomeric configuration occurred during the reaction. The dehydrogenase from retinal pigment epithelium oxidized 11-cis-retinol complexed with cellular retinal-binding protein at pH 8.5 in the presence of NAD. In spite of the ready enzymatic reduction of 11-cis-retinal complexed with cellular retinal-binding protein, the aldehyde function was inaccessible to several chemical reducing agents. Incubation of the complex with NaBH4at pH 7.5 and NaCNBH3or borane dimethylamine at pH 5.5 did not result in reduction of 11-cis-retinal unless the complex had been exposed to white light, a treatment known to produce all-rans-retinal which has little affinity for the binding protein. Liver alcohol dehydrogenase produced only 10% reduction of 11-cis-retinal complexed with cellular retinal-binding protein in 15 min at 37°C when added in amounts which produced about 60% reduction of the uncomplexed retinoid. The results suggest that the interaction between the 11-cis-retinol dehydrogenase and the 11-cis-retinal complexed to cellular retinal-binding protein is a specific one of that the binding protein may function as a substrate carrier for a dehydrogenase.
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DOI：
10.1016/0005-2744(75)90030-3
发表时间：
1970
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
F. Lion;J. Rotmans;F. Daemen;S. L. Bonting
通讯作者：
S. L. Bonting
维生素A的研究：5.视黄烯(1)-维生素A醛的制备。
DOI：
10.1042/bj0420516
发表时间：
1948
期刊：
The Biochemical journal
影响因子：
--
作者：
S. Ball;T. Goodwin;R. A. Morton
通讯作者：
R. A. Morton
自闭症谱系障碍儿童对表达厌恶的面孔的注意偏差：一项探索性研究
影响因子：
4.6
作者：
Zhao X;Zhang P;Fu L;Maes JH
通讯作者：
Maes JH
影响因子：
--
作者：
Hubbard, R.;Brown, P. K.;Bownds, D.
通讯作者：
Bownds, D.
分离的视网膜通过脂质体递送的四黄醇同系物合成视觉色素。
影响因子：
56.9
作者：
S. Uoshikami;GN Noll
通讯作者：
GN Noll