Proinflammatory and Oxidative Stress States Induced by Human Papillomavirus and Chlamydia trachomatis Coinfection Affect Sperm Quality in Asymptomatic Infertile Men.
Proinflammatory and Oxidative Stress States Induced by Human Papillomavirus and Chlamydia trachomatis Coinfection Affect Sperm Quality in Asymptomatic Infertile Men.
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人乳头瘤病毒和沙眼衣原体诱导的促炎和氧化应激状态会影响无症状不育男性的精子质量。
DOI：
10.3390/medicina57090862
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发表时间：
2021-08-24
期刊：
影响因子：
--
通讯作者：
Sánchez-Monroy V
中科院分区：
文献类型：
--
作者：
Pérez-Soto E;Fernández-Martínez E;Oros-Pantoja R;Medel-Flores O;Miranda-Covarrubias JC;Sánchez-Monroy V
背景和目标：探讨人乳头瘤病毒（HPV）、沙眼衣原体（CT）感染及HPV + CT联合感染对无症状不育男性精子质量、炎症和氧化应激（OS）状态的影响。材料和方法：对84例无症状军人不育男性精液进行研究。采用聚合酶链反应（PCR）技术对HPV和CT进行分子检测。根据世界卫生组织指南分析精液参数。通过IL-1β、IL-6和IFN-γ酶联免疫吸附试验（ELISA）评价炎症，通过定量脂质过氧化（LPO）、8-羟基脱氧鸟苷（8-OHdG）和总抗氧化能力（TAC）评价OS。结果如下：84份样本中共有81份（96.4%）为病原体阳性，其中55/81（68%）为HPV阳性，11/81（13.5%）为CT阳性，15/81（18.5%）为HPV + CT合并感染。感染组的精液参数受到影响，包括所有组的pH值升高超过正常范围。在HPV和HPV + CT组中观察到精子形态异常。HPV组细胞因子水平较高，HPV + CT组IL-1β水平最高。CT组未检测到细胞因子。高LPO和8-OHdG水平被发现在所有组与较低的TAC。组间比较显示HPV组中观察到最高OS状态。结论：这些不育男性中的高HPV感染或合并感染（HVP + CT）表明通过诱导促炎状态和OS损害男性生育力。CT感染表明通过促进碱性pH改变OS状态。
Background and Objectives: To investigate the effect of infection with human papillomavirus (HPV) or Chlamydia trachomatis (CT) and HPV + CT coinfection on sperm quality, inflammation, and the state of oxidative stress (OS) in asymptomatic infertile men. Materials and Methods: Semen samples from 84 asymptomatic military infertile men were studied. The polymerase chain reaction (PCR) was used for the molecular detection of HPV and CT. Semen parameters were analyzed according to the World Health Organization guidelines. Inflammation was evaluated by an IL-1β, IL-6, and IFN-γ enzyme-linked immunosorbent assay (ELISA) and OS by the quantification of lipid peroxidation (LPO), 8-hydroxydeoxyguanosine (8-OHdG), and total antioxidant capacity (TAC). Results: A total of 81 of the 84 (96.4%) samples were positives for the pathogens, with 55/81 (68%) being positive for HPV, 11/81 (13.5%) for CT, and 15/81 (18.5%) for HPV + CT coinfection. Seminal parameters were affected in the infected groups, including pH increases above the normal range in all groups. An abnormal sperm morphology was observed in the HPV and HPV + CT groups. Higher cytokine levels were detected in the HPV group and the highest IL-1β level was found in the HPV + CT group. No cytokines were detected in the CT group. High LPO and 8-OHdG levels were found in all groups with a lower TAC. Comparisons between groups showed the highest OS state was observed in the HPV group. Conclusions: High HPV infection or coinfection (HVP + CT) in these infertile men suggest compromising male fertility by inducing a proinflammatory state and OS. Infection with CT suggests an alteration of the state of OS by promoting an alkaline pH.
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影响因子：
2.7
作者：
Reed-Maldonado，Amanda B.;Madden，Kristin C.
通讯作者：
Madden，Kristin C.
DOI：
10.5935/1518-0557.20200020
发表时间：
2020-10-06
期刊：
JBRA assisted reproduction
影响因子：
--
作者：
Goulart ACX;Farnezi HCM;França JPBM;Santos AD;Ramos MG;Penna MLF
通讯作者：
Penna MLF
影响因子：
--
作者：
Damke, Edilson;Kurscheidt, Fabio A.;Consolaro, Marcia E. L.
通讯作者：
Consolaro, Marcia E. L.
影响因子：
2.4
作者：
Tang, Min;Liu, Bian-Jiang;Yin, Chang-Jun
通讯作者：
Yin, Chang-Jun
影响因子：
1.4
作者：
Moazenchi, M.;Totonchi, M.;Meybodi, A. Mohseni
通讯作者：
Meybodi, A. Mohseni