Dynamics of single-cell gene expression.

Dynamics of single-cell gene expression.
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DOI:
10.1038/msb4100110
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发表时间:
2006
影响因子:
9.9
通讯作者:
--
中科院分区:
生物学1区
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--
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细胞行为传统上是通过利用批量规模的方法来研究的,该方法测量细胞群体的平均值。这种群体范围的研究掩盖了单个细胞的行为,并且通常不足以表征细胞异质性起关键作用的生物过程。最近许多研究的一个统一主题是关注单细胞实验技术的开发和利用,这些技术能够探测单个活细胞中的关键生物现象。最近,关于基因表达动力学的新信息已经从单细胞实验中获得,该实验利用荧光报告蛋白的独特能力。
Cellular behavior has traditionally been investigated by utilizing bulk-scale methods that measure average values for a population of cells. Such population-wide studies mask the behavior of individual cells and are often insufficient for characterizing biological processes in which cellular heterogeneity plays a key role. A unifying theme of many recent studies has been a focus on the development and utilization of single-cell experimental techniques that are capable of probing key biological phenomena in individual living cells. Recently, novel information about gene expression dynamics has been obtained from single-cell experiments that draw upon the unique capabilities of fluorescent reporter proteins.
DOI: 10.1038/35014651
发表时间: 2000-06-01
期刊: NATURE
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