Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells.
Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells.
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DOI：
10.1371/journal.pone.0052997
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Herbert BR
中科院分区：
文献类型：
--
作者：
Blaber SP;Hill CJ;Webster RA;Say JM;Brown LJ;Wang SC;Vesey G;Herbert BR
干细胞正日益成为转化研究的焦点，并在人类细胞治疗中发挥着新兴作用。为了支持这些用途，需要用于体内细胞定位和追踪的改进方法。在这项研究中，我们研究了细胞标记对人脂肪间充质干细胞体外功能的影响。我们的研究结果为将来研究植入细胞的命运和寿命提供了基础。特别是，我们研究了两种不同颗粒的影响：微米级（0.9 μm）荧光标记（龙绿色）超顺磁性氧化铁颗粒（M-SPIO颗粒）;和，羧基纳米金刚石的0.25 μm的大小。通过体外形态学、成骨和成脂分化潜能、CD标记物表达、细胞因子分泌谱和细胞内蛋白质组的定量蛋白质组学来评估标记对脂肪源性MSC功能的影响。标记掺入后，干细胞样功能的分化和CD标志物测定未发生改变，未检测到指示应激或毒性的分泌或细胞内蛋白变化。这些体外结果表明，本研究中研究的M-SPIO颗粒和纳米金刚石与MSC具有生物相容性，因此将是用于体内细胞定位和跟踪的合适标记。
Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity. In particular, we investigated the effects of two different particles: micron-sized (∼0.9 µm) fluorescently labeled (Dragon Green) superparamagnetic iron oxide particles (M-SPIO particles); and, carboxylated nanodiamonds of ∼0.25 µm in size. The effects of labeling on the functionality of adipose-derived MSCs were assessed by in vitro morphology, osteogenic and adipogenic differentiation potential, CD marker expression, cytokine secretion profiling and quantitative proteomics of the intra-cellular proteome. The differentiation and CD marker assays for stem-like functionality were not altered upon label incorporation and no secreted or intra-cellular protein changes indicative of stress or toxicity were detected. These in vitro results indicate that the M-SPIO particles and nanodiamonds investigated in this study are biocompatible with MSCs and therefore would be suitable labels for cell localization and tracking in vivo.
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影响因子：
3.3
作者：
Kim, Daehong;Chun, Byoung-Gi;Song, Jihwan
通讯作者：
Song, Jihwan
影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R
影响因子：
38.3
作者：
Chang, Yi-Ren;Lee, Hsu-Yang;Fann, Wunshain
通讯作者：
Fann, Wunshain
影响因子：
4.9
作者：
Guthi JS;Yang SG;Huang G;Li S;Khemtong C;Kessinger CW;Peyton M;Minna JD;Brown KC;Gao J
通讯作者：
Gao J
影响因子：
3.3
作者：
Jendelová, P;Herynek, V;Syková, E
通讯作者：
Syková, E