The chromosome-level genome provides insight into the molecular mechanism underlying the tortuous-branch phenotype of Prunus mume.
The chromosome-level genome provides insight into the molecular mechanism underlying the tortuous-branch phenotype of Prunus mume.
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染色体水平的基因组提供了深入了解梅花弯曲分支表型的分子机制。
DOI：
10.1111/nph.17894
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发表时间：
2022-07
期刊：
影响因子：
9.4
通讯作者：
Zhang, Qixiang
中科院分区：
文献类型：
--
作者：
Zheng, Tangchun;Li, Ping;Zhuo, Xiaokang;Liu, Weichao;Qiu, Like;Li, Lulu;Yuan, Cunquan;Sun, Lidan;Zhang, Zhiyong;Wang, Jia;Cheng, Tangren;Zhang, Qixiang
具有自然扭曲树枝的植物被称为扭曲枝植物，由于其曲折的形状和树枝的自然扭曲，具有极高的观赏价值。梅子是一种重要的木本观赏植物。然而，李属植物这一独特性状的分子机制尚不清楚。在这里，我们利用Oxford纳米孔结合Hi - C支架，构建了一个染色体水平的基因组组装，将237.8 Mb的基因组组装固定在8条假染色体上。分子定年结果表明，梅子属是李属中分化最晚的一种。与细胞分裂、发育和植物激素相关的基因在弯曲分支性状的形成中起重要作用。基于基因表达水平构建了弯曲分支性状的可能调控途径。此外，将候选PmCYCD基因转入拟南芥后，我们发现过表达这些基因的幼苗呈现卷曲的莲座叶片。我们的研究结果为李属新近分化物种的进化史、茎形态的分子基础以及弯曲分支性状的分子机制提供了深入的见解，并突出了多组学在破译梅属植物结构特性方面的应用。另见刘等人对本文的评注，235:8-10。
Plant with naturally twisted branches is referred to as a tortuous‐branch plant, which have extremely high ornamental value due to their zigzag shape and the natural twisting of their branches. Prunus mume is an important woody ornamental plant. However, the molecular mechanism underlying this unique trait in Prunus genus is unknown. Here, we present a chromosome‐level genome assembly of the cultivated P. mume var. tortuosa created using Oxford Nanopore combined with Hi‐C scaffolding, which resulted in a 237.8 Mb genome assembly being anchored onto eight pseudochromosomes. Molecular dating indicated that P. mume is the most recently differentiated species in Prunus. Genes associated with cell division, development and plant hormones play essential roles in the formation of tortuous branch trait. A putative regulatory pathway for the tortuous branch trait was constructed based on gene expression levels. Furthermore, after transferring candidate PmCYCD genes into Arabidopsis thaliana, we found that seedlings overexpressing these genes exhibited curled rosette leaves. Our results provide insights into the evolutionary history of recently differentiated species in Prunus genus, the molecular basis of stem morphology, and the molecular mechanism underlying the tortuous branch trait and highlight the utility of multi‐omics in deciphering the properties of P. mume plant architecture. See also the Commentary on this article by Liu et al., 235: 8–10.
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