Three high-lysine mutations control the level of ATP-binding HSP70-like proteins in the maize endosperm.
Three high-lysine mutations control the level of ATP-binding HSP70-like proteins in the maize endosperm.
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三个高赖氨酸突变控制玉米胚乳中 ATP 结合 HSP70 样蛋白的水平。
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
F. Salamini
中科院分区：
文献类型：
--
作者：
A. Marocco;A. Santucci;S. Cerioli;M. Motto;N. Di Fonzo;R. Thompson;F. Salamini
玉米种子储存蛋白的合成和沉积受到几种显性和隐性突变体的影响。研究了降低胚乳中玉米醇溶蛋白水平的三个独立突变：floury-2 (fl2)、缺陷胚乳-B30 (De-B30) 和 Mucronate (Mc) 的影响。这些突变还控制 b-70 的水平，b-70 是一种与蛋白体结合的多肽，通过二维凝胶电泳可将其分为两种亚型 b-70I 和 b-70II。两种亚型在 fl2 中均过表达 10 倍；然而，De-B30 和 Mc 中仅存在 b-70I，其 B-70 亚型总量比野生型高出五倍。 b-70I 和 b-70II 都类似于热休克蛋白 (HSP70)，因为它们结合 ATP，与抗 HSP 抗体交叉反应，并且与 HSP70 具有 N 端序列同源性。所有位于玉米蛋白体内的 b-70 特征都是类伴侣热休克蛋白的典型特征。第三种蛋白质 b-70III 大小与 b-70I 和 b-70II 相似，但酸性稍强，也结合 ATP 并与相同的抗体反应，为胚乳提取物中存在胞质伴侣蛋白样蛋白提供证据。 b-70III 的水平并未因所研究的突变而改变。结果表明，三种突变对玉米醇溶蛋白积累的抑制作用可能是通过改变涉及 b-70I 和 b-70II 的玉米醇溶蛋白转运或玉米醇溶蛋白组装过程来介导的。
The synthesis and deposition of seed storage proteins in maize are affected by several dominant and recessive mutants. The effect of three independent mutations, floury-2 (fl2), Defective endosperm-B30 (De-B30), and Mucronate (Mc), that reduce zein level in the endosperm were investigated. These mutations also control the level of b-70, a polypeptide bound to protein bodies, which is separable into the two isoforms b-70I and b-70II by two-dimensional gel electrophoresis. Both isoforms are overexpressed 10-fold in fl2; however, only b-70I is present in De-B30 and Mc, which contain an amount of total b-70 isoforms fivefold higher than in the wild type. Both b-70I and b-70II resemble heat shock protein (HSP70) in that they bind ATP, cross-react with anti-HSP antibodies, and have N-terminal sequence homology to HSP70. All maize protein body-located b-70 characteristics are typical of those of chaperone-like HSPs. A third protein, b-70III, similar in size to but slightly more acidic than b-70I and b-70II, also binds ATP and reacts with the same antibody, providing evidence for the presence in endosperm extracts of a cytosolic chaperone-like protein. The level of b-70III was not altered by the mutations studied. The results suggested that the repression effect of the three mutations on zein accumulation may be mediated by the alteration of a zein transport or zein assembly process involving b-70I and b-70II.
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DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
哺乳动物 70,000 道尔顿应激蛋白的快速纯化：蛋白质对核苷酸的亲和力。
影响因子：
5.3
作者：
Welch,WJ;Feramisco,JR
通讯作者：
Feramisco,JR
影响因子：
56.9
作者：
BECKMANN, RP;MIZZEN, LA;WELCH, WJ
通讯作者：
WELCH, WJ
影响因子：
56.9
作者：
KURTZ, S;ROSSI, J;LINDQUIST, S
通讯作者：
LINDQUIST, S
DOI：
10.1073/pnas.87.1.46
发表时间：
1990-01-01
影响因子：
11.1
作者：
SCHMIDT, RJ;BURR, FA;BURR, B
通讯作者：
BURR, B