The effects of dietary (n-3) fatty acid supplementation on lipid dynamics and composition in rat lymphocytes and liver microsomes.
The effects of dietary (n-3) fatty acid supplementation on lipid dynamics and composition in rat lymphocytes and liver microsomes.
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膳食 (n-3) 脂肪酸补充剂对大鼠淋巴细胞和肝微粒体脂质动态和组成的影响。
DOI：
10.1016/0005-2736(86)90454-2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Smith,AD
中科院分区：
文献类型：
--
作者：
Conroy,DM;Stubbs,CD;Belin,J;Pryor,CL;Smith,AD
大鼠被喂食缺乏（n-3）脂肪酸（橄榄油补充剂）或高（n-3）脂肪酸（鱼油补充剂）的饮食10天。在喂食鱼油饲料的动物的脾淋巴细胞和肝微粒体中，与喂食橄榄油饲料时的最低水平相比，获得了相对较高水平的（n-3）二十碳五烯酸（20：5）、二十二碳五烯酸（22：5）和二十二碳六烯酸（22：6）。当通过测量二苯基己三烯的荧光各向异性来检查平均脂质运动特性时，在喂食两种饮食的动物的完整肝微粒体之间没有发现显著差异。然而，当从鱼油喂养动物的微粒体中分离出磷脂酰胆碱囊泡（21.4%（n-3）脂肪酸）时，检测脂质运动时，荧光各向异性显著低于橄榄油喂养动物的相应磷脂酰胆碱（5.6%（n-3）脂肪酸），表明在存在较高水平（n-3）脂肪酸的情况下，双层更加无序或流动。补充鱼油后，磷脂酰乙醇胺（n-3）脂肪酸也升高（占总脂肪酸的41.3%），而橄榄油补充后的水平（21.4%）。鱼油补充剂的主要作用是用（n-3）脂肪酸取代（n-6）花生四烯酸，当使用合成脂质脂质、1-棕榈酰-2-花生四烯酸（n-6）或二十二碳六烯酰（n-3）-磷脂酰胆碱的脂质体进行“建模”时，发现脂质运动特性存在显着差异，其中二十二碳六烯酸提供了更加无序或流动的脂质环境。因此，尽管高度不饱和（n-3）脂肪酸水平的增加可以显著降低脂质有序性/流动性，但膜结构域组织和/或磷脂分子种类组成的改变有效地补偿了这些变化，至少就完整膜中的平均脂质运动特性而言。
Rats were fed diets devoid of (n− 3) fatty acids (olive oil supplementation) or high in (n− 3) fatty acids (fish oil supplementation) for a period of 10 days. In spleen lymphocytes and liver microsomes derived from animals fed fish oil diets, relatively high levels of (n− 3) eicosapentaenoic (20: 5), docosapentaenoic (22: 5) and docosahexaenoic acids (22: 6) were obtained compared to minimal levels when fed the olive oil diet. When the average lipid motional properties were examined by measuring the fluorescence anisotropy of diphenylhexatriene, no significant different was found between intact liver microsomes from animals fed the two diets. However, when lipid motion was examined in vesicles of phosphatidylcholine, isolated from the microsomes from fish oil fed animals (21.4%(n− 3) fatty acids), the fluorescence anisotropy was significantly less than the corresponding phosphatidylcholine from olive oil fed animals (5.6%(n− 3) fatty acids), indicating a more disordered or fluid bilayer in the presence of higher levels of (n− 3) fatty acids. Phosphatidylethanolamine (n− 3) fatty acids were also elevated after fish oil supplementation (41.3% of total fatty acids), compared to the level after olive oil supplementation (21.4%). The major effect of the fish oil supplementation was a replacement of (n− 6) arachidonic acid by the (n− 3) fatty acids and when this was ‘modeled’, using liposomes of synthetic lipids, 1-palmitoyl-2-arachidonyl (n− 6) or docosahexaenoyl (n− 3)-phosphatidylcholine, significant differences in lipid motional properties were found, with the docosahexaenoate conferring a more disordered or fluid lipid environment. Thus it appears that although lipid order/fluidity can be significantly decreased by increases in the highly unsaturated (n− 3) fatty acid levels, alterations in membrane domain organization and/or phospholipid molecular species composition effectively compensated for the changes, at least as far as average lipid motional properties in the intact membranes was concerned.
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作者：
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10.1016/s0021-9673(01)92081-3
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