Higher prevalence of Epstein-Barr virus DNA in deeper periodontal pockets of chronic periodontitis in Japanese patients.
Higher prevalence of Epstein-Barr virus DNA in deeper periodontal pockets of chronic periodontitis in Japanese patients.
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DOI：
10.1371/journal.pone.0071990
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ogata Y
中科院分区：
文献类型：
--
作者：
Kato A;Imai K;Ochiai K;Ogata Y
牙周炎是由龈下感染引起的一种复杂的慢性炎症性疾病，是人类最常见的微生物疾病之一。虽然传统的牙周炎微生物学研究主要集中在牙龈卟啉单胞菌等可能的细菌上，但疱疹病毒被认为与牙周炎的发病机制有关，因为单靠细菌病原学不能充分解释各种临床方面。在这项研究中，我们首次在日本慢性牙周炎患者的牙周袋中发现了更多的EB病毒(EBV)DNA。对85例牙周探诊深度分别为≤3 mm(浅)和≥5 mm(深)的慢性牙周炎患者的龈下标本进行套式聚合酶链式反应。EB病毒DNA在PD部位较深的患者(66%)的检出频率高于PD部位较浅的患者(48%)或健康对照组(45%)。PD部位较深的患者(40%)的EBVDNA和牙龈假单胞菌的共存显著高于PD部位较浅的患者(14%)或健康对照组(13%)。虽然牙周炎的临床指标没有差异，但PD部位较深的患者EBV DNA的优势比为2.36，是PD部位较浅的患者的2.07倍。有趣的是，同时存在EB病毒DNA和牙龈假单胞菌时，患慢性牙周炎(PD≥5 mm)的几率比单独存在EBVDNA或P.givalis时更高。此外，我们还观察到EBV编码的小RNA(EBER)在人牙龈组织中的阳性细胞中。这些结果表明，EBV DNA可能是导致日本患者慢性牙周炎的一个致病因素。
Periodontitis, a complex chronic inflammatory disease caused by subgingival infection, is among the most prevalent microbial diseases in humans. Although traditional microbiological research on periodontitis has focused on putative bacteria such as Porphyromonas gingivalis, the herpes virus is proposed to be involved in the pathogenesis of periodontitis because bacterial etiology alone does not adequately explain various clinical aspects. In this study, we established for the first time, more Epstein–Barr virus (EBV) DNA is found deeper in periodontal pockets of chronic periodontitis in Japanese patients. Subgingival samples were collected from 85 patients with chronic periodontitis having two periodontal sites with probing depths (PD) of ≤3 mm (shallow) or ≥5 mm (deep) and were subjected to a nested polymerase chain reaction. EBV DNA was more frequently detected in patients with deeper PD sites (66%) than in those with shallow PD sites (48%) or healthy controls (45%). Coexistence of EBV DNA and P. gingivalis was significantly higher in patients with deeper PD sites (40%) than in those with shallow PD sites (14%) or healthy controls (13%). Although no difference in clinical index for periodontitis, the odds ratio of EBV DNA in patients with deeper PD sites was 2.36, which was 2.07-fold higher than that in those with shallow PD sites. Interestingly, the odds of acquiring chronic periodontitis (PD ≥5 mm) were higher in the presence of both EBV DNA and P. gingivalis compared with either EBV DNA or P. gingivalis only. In addition, we also observed that EBV-encoded small RNA (EBER) in positive cells of human gingival tissues. These results would suggest that EBV DNA may serve as a pathogenic factor leading to chronic periodontitis among Japanese patients.
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