Temporal response dynamics of Drosophila olfactory sensory neurons depends on receptor type and response polarity.
Temporal response dynamics of Drosophila olfactory sensory neurons depends on receptor type and response polarity.
复制标题
果蝇的时间反应动力学，嗅觉感觉神经元取决于受体类型和反应极性。
DOI：
10.3389/fncel.2012.00054
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发表时间：
2012
影响因子：
5.3
通讯作者：
Olsson SB
中科院分区：
文献类型：
--
作者：
Getahun MN;Wicher D;Hansson BS;Olsson SB
昆虫嗅觉感觉神经元(OSN)表达一系列不同蛋白质家族的受体，即离子受体(IR)、味觉受体(GR)和气味受体(OR)。众所周知，昆虫暴露在过多的气味分子中，在动荡的自然条件下，气味分子在空间和时间上都有很大的变化。除了不同的配体特异性，这些不同的受体也可能为嗅觉系统提供更大范围的时间动力学和敏感性。为了验证这一点，我们用不同的刺激持续时间(10-2000毫秒)和以不同频率(1-10赫兹)重复关键配体的刺激脉冲来挑战不同的果蝇OSN。我们的结果表明，与表达IR和Gr21a的OSNs相比，表达OR的OSNs对短刺激的反应更快、更敏感。此外，表达OR的OSNs可以对高达5 Hz的兴奋性配体的重复刺激做出反应，而表达IR的OSNs需要大约5倍的刺激时间和/或更高的浓度才能对类似的刺激持续时间和频率做出反应。然而，与OR-和Gr21a-OSN不同，表达IR的OSN不表现出对更长刺激的适应。OR和IR表达的OSN也不能像兴奋性配体那样快速地分辨抑制性配体的重复脉冲。这些差异与受体表达所处的受体周围环境无关，表明特定OSN表达的受体既影响其敏感性，也影响其对瞬时、间歇性化学刺激的反应。表达OR的OSN在分辨低剂量、间歇性刺激方面表现得更好，而表达IR的OSN对长时间气味脉冲的反应更准确。这种多样性增加了昆虫嗅觉系统对自然环境中不同时空信号的反应能力。
Insect olfactory sensory neurons (OSN) express a diverse array of receptors from different protein families, i.e. ionotropic receptors (IR), gustatory receptors (GR) and odorant receptors (OR). It is well known that insects are exposed to a plethora of odor molecules that vary widely in both space and time under turbulent natural conditions. In addition to divergent ligand specificities, these different receptors might also provide an increased range of temporal dynamics and sensitivities for the olfactory system. To test this, we challenged different Drosophila OSNs with both varying stimulus durations (10–2000 ms), and repeated stimulus pulses of key ligands at various frequencies (1–10 Hz). Our results show that OR-expressing OSNs responded faster and with higher sensitivity to short stimulations as compared to IR- and Gr21a-expressing OSNs. In addition, OR-expressing OSNs could respond to repeated stimulations of excitatory ligands up to 5 Hz, while IR-expressing OSNs required ~5x longer stimulations and/or higher concentrations to respond to similar stimulus durations and frequencies. Nevertheless, IR-expressing OSNs did not exhibit adaptation to longer stimulations, unlike OR- and Gr21a-OSNs. Both OR- and IR-expressing OSNs were also unable to resolve repeated pulses of inhibitory ligands as fast as excitatory ligands. These differences were independent of the peri-receptor environment in which the receptors were expressed and suggest that the receptor expressed by a given OSN affects both its sensitivity and its response to transient, intermittent chemical stimuli. OR-expressing OSNs are better at resolving low dose, intermittent stimuli, while IR-expressing OSNs respond more accurately to long-lasting odor pulses. This diversity increases the capacity of the insect olfactory system to respond to the diverse spatiotemporal signals in the natural environment.
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影响因子：
9.8
作者：
Benton, Richard;Sachse, Silke;Michnick, Stephen W;Vosshall, Leslie B
通讯作者：
Vosshall, Leslie B
影响因子：
2.8
作者：
Dolzer, J;Fischer, K;Stengl, M
通讯作者：
Stengl, M
DOI：
10.1073/pnas.0505340102
发表时间：
2005-09-27
影响因子：
11.1
作者：
Ishida, Y;Leal, WS
通讯作者：
Leal, WS
影响因子：
16.2
作者：
Goulding, SE;zur Lage, P;Jarman, AP
通讯作者：
Jarman, AP
影响因子：
25
作者：
Louis, Matthieu;Huber, Thomas;Vosshall, Leslie B.
通讯作者：
Vosshall, Leslie B.