Analysis of microRNAs Expression Profiles in Madin-Darby Bovine Kidney Cells Infected With Caprine Parainfluenza Virus Type 3.
Analysis of microRNAs Expression Profiles in Madin-Darby Bovine Kidney Cells Infected With Caprine Parainfluenza Virus Type 3.
复制标题
感染山羊副流感病毒 3 型的 Madin-Darby 牛肾细胞中 microRNA 表达谱分析
DOI：
10.3389/fcimb.2018.00093
复制
发表时间：
2018
影响因子：
5.7
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Li J;Mao L;Li W;Hao F;Zhong C;Zhu X;Ji X;Yang L;Zhang W;Liu M;Jiang J
山羊副流感病毒3型（CPIV 3）是一种新出现的呼吸道病原体，感染年轻和成年山羊，并于2013年在中国东部被发现。细胞microRNAs（miRNAs）是病毒与宿主相互作用的重要调节因子。为了阐明miRNAs在CPIV 3感染MDBK细胞中的作用。在这项研究中，我们进行了高通量测序技术来分析小RNA库在CPIV 3感染和模拟感染MDBK细胞。结果显示，CPIV 3感染MDBK细胞后，共有249个已知和152个新的候选miRNA差异表达，预测的靶基因分别为22，981个和22，572个。此外，RT-qPCR分析用于进一步确认这些差异表达的miRNAs中的13个及其mRNA靶的表达模式。功能注释分析显示，这些上调和下调的靶基因主要涉及MAPK信号通路、Jak-STAT信号通路、Toll样受体信号通路、p53信号通路、黏着斑、NF-κ B信号通路和凋亡等。我们的发现提供了有关miRNA表达谱的信息，以应对CPIV 3感染，并为确定抗CPIV 3的抗病毒治疗的潜在候选人提供了线索。
Caprine parainfluenza virus type 3 (CPIV3) is a newly emerging pathogenic respiratory agent infecting both young and adult goats, and it was identified in eastern China in 2013. Cellular microRNAs (miRNAs) have been reported to be important modulators of the intricate virus-host interactions. In order to elucidate the role of miRNAs in madin-darby bovine kidney (MDBK) cells during CPIV3 infection. In this study, we performed high-throughput sequencing technology to analyze small RNA libraries in CPIV3-infected and mock-infected MDBK cells. The results showed that a total of 249 known and 152 novel candidate miRNAs were differentially expressed in MDBK cells after CPIV3 infection, and 22,981 and 22,572 target genes were predicted, respectively. In addition, RT-qPCR assay was used to further confirm the expression patterns of 13 of these differentially expressed miRNAs and their mRNA targets. Functional annotation analysis showed these up- and downregulated target genes were mainly involved in MAPK signaling pathway, Jak-STAT signaling pathway, Toll-like receptor signaling pathway, p53 signaling pathway, focal adhesion, NF-kappa B signaling pathway, and apoptosis, et al. To our knowledge, this is the first report of the comparative expression of miRNAs in MDBK cells after CPIV3 infection. Our finding provides information concerning miRNAs expression profile in response to CPIV3 infection, and offers clues for identifying potential candidates for antiviral therapies against CPIV3.
登录
查看更多内容
影响因子：
3.3
作者：
Li W;Mao L;Cheng S;Wang Q;Huang J;Deng J;Wang Z;Zhang W;Yang L;Hao F;Ding Y;Sun Y;Wei J;Jiang P;Jiang J
通讯作者：
Jiang J
慢病毒介导的牛bta-miR-29b过表达通过直接靶向Madin-Darby牛肾细胞中的ATG14和ATG9A干扰牛病毒性腹泻病毒复制和病毒感染相关的自噬
影响因子：
3.8
作者：
Fu, Qiang;Shi, Huijun;Chen, Chuangfu
通讯作者：
Chen, Chuangfu
影响因子：
3.8
作者：
Horwood, Paul Francis;Gravel, Jennifer Lillian;Mahony, Timothy John
通讯作者：
Mahony, Timothy John
bta-miR-29b启动子区DNA甲基化在调节MDBK细胞中miR-29b表达和牛病毒性腹泻病毒NADL复制中的作用
影响因子：
2.7
作者：
Fu, Qiang;Shi, Huijun;Chen, Chuangfu
通讯作者：
Chen, Chuangfu
影响因子：
4.6
作者：
Jia X;Bi Y;Li J;Xie Q;Yang H;Liu W
通讯作者：
Liu W