Arabidopsis BREVIPEDICELLUS interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA to regulate KNAT2 and KNAT6 expression in control of inflorescence architecture.
Arabidopsis BREVIPEDICELLUS interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA to regulate KNAT2 and KNAT6 expression in control of inflorescence architecture.
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拟南芥 BREVIPEDICELLUS 与 SWI2/SNF2 染色质重塑 ATP 酶 BRAHMA 相互作用，调节 KNAT2 和 KNAT6 表达，控制花序结构
DOI：
10.1371/journal.pgen.1005125
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发表时间：
2015-03
期刊：
影响因子：
4.5
通讯作者：
Wu K
中科院分区：
文献类型：
--
作者：
Zhao M;Yang S;Chen CY;Li C;Shan W;Lu W;Cui Y;Liu X;Wu K
BREVIPEDICELLUS（BP或KNAT 1）是拟南芥中的一个类KNOTTED 1同源盒（KNOX）转录因子，通过负调控KNAT 2和KNAT 6两个类KNOX基因来形成正常的花序结构。然而，BP介导的转录调控的分子机制仍不清楚。在这项研究中，我们发现BP直接与SWI 2/SNF 2染色质重塑ATP酶BRAHMA（BRM）在体外和体内相互作用。功能丧失的BRM突变体表现出花序结构缺陷，具有簇生花序，水平取向的花梗，短花梗和节间，与bp突变体的表型相似。此外，在brm-3、bp-9和brm-3 bp-9双突变体中，KNAT 2和KNAT 6的转录水平升高。在brm-3、bp-9和brm-3 bp-9双突变体中检测到组蛋白H3赖氨酸4三甲基化（H3 K4 me 3）水平增加。此外，BRM和BP共同靶向KNAT 2和KNAT 6基因，并且BP是BRM与KNAT 2和KNAT 6结合所必需的。总之，我们的研究结果表明，BP与染色质重塑因子BRM相互作用，调节KNAT 2和KNAT 6的表达，控制花序结构。 BP是一种I类KNOX转录因子，通过抑制两种KNOX基因KNAT 2和KNAT 6的表达来控制正常的花序结构发育。在这项研究中，我们发现拟南芥BP直接与SWI 2/SNF 2染色质重塑ATP酶BRM相互作用。BRM和BP突变体表现出相似的花序结构缺陷，具有簇生花序、水平取向的花梗、短花梗和节间。此外，BP和BRM共同靶向KNAT 2和KNAT 6基因并抑制其表达。这项工作揭示了一个新的调节机制，BP协会与BRM在控制花序结构的发展。
BREVIPEDICELLUS (BP or KNAT1), a class-I KNOTTED1-like homeobox (KNOX) transcription factor in Arabidopsis thaliana, contributes to shaping the normal inflorescence architecture through negatively regulating other two class-I KNOX genes, KNAT2 and KNAT6. However, the molecular mechanism of BP-mediated transcription regulation remains unclear. In this study, we showed that BP directly interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA (BRM) both in vitro and in vivo. Loss-of-function BRM mutants displayed inflorescence architecture defects, with clustered inflorescences, horizontally orientated pedicels, and short pedicels and internodes, a phenotype similar to the bp mutants. Furthermore, the transcript levels of KNAT2 and KNAT6 were elevated in brm-3, bp-9 and brm-3 bp-9 double mutants. Increased histone H3 lysine 4 tri-methylation (H3K4me3) levels were detected in brm-3, bp-9 and brm-3 bp-9 double mutants. Moreover, BRM and BP co-target to KNAT2 and KNAT6 genes, and BP is required for the binding of BRM to KNAT2 and KNAT6. Taken together, our results indicate that BP interacts with the chromatin remodeling factor BRM to regulate the expression of KNAT2 and KNAT6 in control of inflorescence architecture. BP is a class-I KNOX transcription factor that controls normal inflorescence architecture development by repressing the expression of two KNOX genes, KNAT2 and KNAT6. In this study, we showed that Arabidopsis BP directly interacts with the SWI2/SNF2 chromatin remodeling ATPase BRM. brm and bp mutants displayed similar inflorescence architecture defects, with clustered inflorescences, horizontally orientated pedicels, and short pedicels and internodes. Furthermore, BP and BRM co-target to KNAT2 and KNAT6 genes and repress their expression. This work reveals a new regulatory mechanism that BP associates with BRM in control of inflorescence architecture development.
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