The effect of culture density and proliferation rate on the expression of ouabain-sensitive Na/K ATPase pumps in cultured human retinal pigment epithelium.
The effect of culture density and proliferation rate on the expression of ouabain-sensitive Na/K ATPase pumps in cultured human retinal pigment epithelium.
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培养密度和增殖率对培养的人视网膜色素上皮中哇巴因敏感的Na/K ATP酶泵表达的影响。
DOI：
10.1016/0014-4827(91)90353-v
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发表时间：
1991
影响因子：
3.7
通讯作者：
Brzeski,CM
中科院分区：
文献类型：
--
作者：
Burke,JM;Jaffe,GJ;Brzeski,CM
哇巴因敏感的Na K ATP酶泵的数量和活性在体外表达的许多细胞类型，包括人视网膜色素上皮细胞（RPE），已被证明随着培养密度的增加而下降。随着培养物变得致密，细胞增殖也下降，因此尚不清楚泵数是否受到细胞增殖或培养物融合的调节。通过将RPE培养物暴露于各种饲养方案，使用含有或不含血清的培养基，可以产生具有一系列培养密度和生长速率的RPE培养物。通过定量[3 H]胸苷掺入分析这些细胞的增殖活性，通过测量特异性[3 H]哇巴因结合分析Na K ATP酶泵数。结果表明，泵数调制的培养密度，并进一步，密度依赖性调节泵数需要血清。虽然培养物生长的密度依赖性调节也需要血清，但细胞增殖和泵数量似乎不相关;生长速率显著不同的相似密度的培养物具有相似的泵数量。用[3 H]胸苷和[3 H]哇巴因双重标记的细胞放射自显影的定性检查进一步支持了在增殖细胞中不一定发现泵数量升高的观点。[3 H]胸腺嘧啶标记细胞核的细胞周期中，[3 H]哇巴因的标记没有显著增加。然而，[3 H]哇巴因标记，作为泵位点数量和分布的指标，确实在RPE群体中的细胞之间以及在单个细胞内变化。后一种观察结果表明，稀疏培养物中的非极化RPE细胞可能对离子调节具有区域性不同的要求。
The number and activity of ouabain-sensitive Na K ATPase pumps expressed by many cell types in vitro, including human retinal pigment epithelial cells (RPE), have been shown to decline with increasing culture density. Cell proliferation also declined as cultures became dense so it was unclear if pump number was modulated by cell proliferation or culture confluency. By exposing RPE cultures to various feeding regimens, using culture medium containing or lacking serum, it was possible to produce RPE cultures with a range of culture densities and growth rates. These were analyzed for proliferative activity by quantifying [3 H] thymidine incorporation and for Na K ATPase pump number by measuring specific [3 H] ouabain binding. The results suggest that pump number is modulated by culture density and, further, that the density-dependent regulation of pump number requires serum. Although density-dependent modulation of culture growth is also serum requiring, cell proliferation and pump number did not appear to be related; cultures of similar density which differed significantly in growth rate had similar numbers of pumps. The view that elevated numbers of pumps were not necessarily found in proliferating cells was further supported by qualitative examination of radioautographs of cells dually labeled with [3 H] thymidine and [3 H] ouabain. Cycling cells which had [3 H] thymidine-labeled nuclei did not have notably higher labeling with [3 H] ouabain. However,[3 H] ouabain labeling, as an indicator of pump site number and distribution, did vary among cells in an RPE population and also within individual cells. This latter observation suggests that unpolarized RPE cells in sparse cultures may have regionally different requirements for ionic regulation.
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培养牛角膜内皮细胞中 Na、K-ATP 酶泵的表征。
影响因子：
3.4
作者：
N. Savion;N. Farzame
通讯作者：
N. Farzame
体外脂肪细胞分化过程中葡萄糖转运蛋白和(Na,K)-腺苷三磷酸酶的胰岛素反应性的发展。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Resh,MD
通讯作者：
Resh,MD
DOI：
10.1083/jcb.85.2.377
发表时间：
1980-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Vogel A;Ross R;Raines E
通讯作者：
Raines E
影响因子：
56.9
作者：
ROZENGURT, E
通讯作者：
ROZENGURT, E
生长细胞和接触抑制细胞中细胞内钾浓度和钾通量之间的关系。
DOI：
--
发表时间：
1975
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
B. Ducouret;L. Lelièvre;A. Paraf;A. Kepes
通讯作者：
A. Kepes