Synthetic Control of Tertiary Helical Structures in Short Peptides.
Synthetic Control of Tertiary Helical Structures in Short Peptides.
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DOI：
10.1021/jacs.8b10082
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发表时间：
2018-11-28
影响因子：
15
通讯作者：
Arora PS
中科院分区：
文献类型：
--
作者：
Wuo MG;Hong SH;Singh A;Arora PS
螺旋状的二级和三级基序通常被观察到作为天然和工程蛋白支架的结合表位。虽然已经描述了几种策略来限制α-螺旋或在合成仿制品中再现其结合属性，但模仿三级螺旋基序的一般策略仍处于起步阶段。我们最近描述了一种开发螺旋二聚体的合成策略。化学。学报，2015,37(3):11618-11621。我们发现用共价键取代螺旋间盐桥可以稳定短序列中的反平行基序。这里我们证明了该方法可以推广到得到反平行和平行二聚体以及三聚体基序。螺旋稳定需要明智地设计交联剂以及最佳的螺旋间疏水填料。我们预计这些模拟物将提供新的生物功能调节剂类别。
Helical secondary and tertiary motifs are commonly observed as binding epitopes in natural and engineered protein scaffolds. While several strategies have been described to constrain α-helices or reproduce their binding attributes in synthetic mimics, general strategies to mimic tertiary helical motifs remain in their infancy. We recently described a synthetic strategy to develop helical dimers (J. Am. Chem. Soc. 2015, 137, 11618–11621). We found that replacement of an interhelical salt bridge with a covalent bond can stabilize antiparallel motifs in short sequences. Here we show that the approach can be generalized to obtain antiparallel and parallel dimers as well as trimer motifs. Helical stabilization requires judiciously designed crosslinkers as well as optimal interhelical hydrophobic packing. We anticipate that these mimics would afford new classes of modulators of biological function.
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影响因子：
7.8
作者：
Henchey, Laura K.;Jochim, Andrea L.;Arora, Paramjit S.
通讯作者：
Arora, Paramjit S.
影响因子：
64.8
作者：
Bhardwaj, Gaurav;Mulligan, Vikram Khipple;Bahl, Christopher D.;Gilmore, Jason M.;Harvey, Peta J.;Cheneval, Olivier;Buchko, Garry W.;Pulavarti, Surya V. S. R. K.;Kaas, Quentin;Eletsky, Alexander;Huang, Po-Ssu;Johnsen, William A.;Greisen, Per Jr;Rocklin, Gabriel J.;Song, Yifan;Linsky, Thomas W.;Watkins, Andrew;Rettie, Stephen A.;Xu, Xianzhong;Carter, Lauren P.;Bonneau, Richard;Olson, James M.;Coutsias, Evangelos;Correnti, Colin E.;Szyperski, Thomas;Craik, David J.;Baker, David
通讯作者：
Baker, David
影响因子：
16.6
作者：
Desmet, Johan;Verstraete, Kenneth;Bloch, Yehudi;Lorent, Eric;Wen, Yurong;Devreese, Bart;Vandenbroucke, Karen;Loverix, Stefan;Hettmann, Thore;Deroo, Sabrina;Somers, Klaartje;Henderikx, Paula;Lasters, Ignace;Savvides, Savvas N.
通讯作者：
Savvides, Savvas N.
DOI：
10.1034/j.1399-3011.2001.10972.x
发表时间：
2001-12-01
期刊：
JOURNAL OF PEPTIDE RESEARCH
影响因子：
--
作者：
Litowski, JR;Hodges, RS
通讯作者：
Hodges, RS
影响因子：
15
作者：
Bilgiçer, B;Fichera, A;Kumar, K
通讯作者：
Kumar, K