The differentiation status of primary gonadal germ cell tumors correlates inversely with telomerase activity and the expression level of the gene encoding the catalytic subunit of telomerase.
The differentiation status of primary gonadal germ cell tumors correlates inversely with telomerase activity and the expression level of the gene encoding the catalytic subunit of telomerase.
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DOI：
10.1186/1471-2407-2-32
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发表时间：
2002-11-29
期刊：
影响因子：
3.8
通讯作者：
Miller K
中科院分区：
文献类型：
--
作者：
Schrader M;Burger AM;Müller M;Krause H;Straub B;Schostak M;Schulze W;Lauke H;Miller K
在生殖细胞、干细胞和肿瘤细胞中可以检测到核糖核蛋白端粒酶的活性。端粒酶的主要成分之一是人端粒酶逆转录酶（hTERT），它编码端粒酶的催化亚基。在这里，我们研究端粒酶活性和hTERT基因表达与原发性睾丸生殖细胞肿瘤（TGCT）分化状态的相关性。通过定量端粒酶 PCR ELISA 检测端粒酶活性 (TA)，并通过在线 RT-PCR 定量 42 个原发性睾丸生殖细胞肿瘤中的 hTERT mRNA 表达。对照组由不育患者的良性睾丸活检组成。在所有检查的未分化 TGCT 和含有生殖细胞的良性睾丸组织样本中均检测到高水平的端粒酶活性和 hTERT 表达。相比之下，分化的畸胎瘤和没有生殖细胞的睾丸对照组织（仅支持细胞综合征）没有表现出端粒酶活性，并且只有极少的 hTERT 表达。这些发现证明端粒酶活性水平和hTERT mRNA表达与生殖细胞肿瘤的分化状态之间存在反比关系。端粒酶活性和 hTERT mRNA 表达的量化能够对生殖细胞肿瘤进行新的分子诊断亚分类，描述其增殖潜力和分化状态。
The activity of the ribonucleoprotein enzyme telomerase is detectable in germ, stem and tumor cells. One major component of telomerase is human telomerase reverse transcriptase (hTERT), which encodes the catalytic subunit of telomerase. Here we investigate the correlation of telomerase activity and hTERT gene expression and the differentiation status of primary testicular germ cell tumors (TGCT). Telomerase activity (TA) was detected by a quantitative telomerase PCR ELISA, and hTERT mRNA expression was quantified by online RT-PCR in 42 primary testicular germ cell tumors. The control group consisted of benign testicular biopsies from infertile patients. High levels of telomerase activity and hTERT expression were detected in all examined undifferentiated TGCTs and in the benign testicular tissue specimens with germ cell content. In contrast, differentiated teratomas and testicular control tissue without germ cells (Sertoli-cell-only syndrome) showed no telomerase activity and only minimal hTERT expression. These findings demonstrate an inverse relationship between the level of telomerase activity and hTERT mRNA expression and the differentiation state of germ cell tumors. Quantification of telomerase activity and hTERT mRNA expression enables a new molecular-diagnostic subclassification of germ cell tumors that describes their proliferation potential and differentiation status.
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影响因子：
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作者：
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通讯作者：
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