Neurons identified by NeuN/Fox-3 immunoreactivity have a novel distribution in the hamster and mouse suprachiasmatic nucleus.
Neurons identified by NeuN/Fox-3 immunoreactivity have a novel distribution in the hamster and mouse suprachiasmatic nucleus.
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DOI：
10.1016/j.brainres.2011.09.015
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发表时间：
2011-11-03
期刊：
影响因子：
2.9
通讯作者：
Studholme KM
中科院分区：
文献类型：
--
作者：
Morin LP;Hefton S;Studholme KM
视交叉上核（SCN）具有几种跨物种共享的结构特征和细胞表型。在这里，我们描述了一个新的功能SCN解剖，是在仓鼠和小鼠。从固定的脑中获得通过SCN的冷冻切片，并使用已知专门识别神经元的小鼠单克隆抗体对神经元核蛋白（NeuN-IR）的免疫反应性进行染色。NeuN-IR没有识别所有SCN神经元，因为内侧NeuNIR神经元通常不存在。在仓鼠中，NeuN-IR细胞存在于吻侧，分散在核的背侧。在更尾部，NeuN-IR细胞主要但不完全分散在外侧和背外侧边界内。在SCN的中尾侧水平，一组密集的NeuN-IR细胞从背外侧缘腹内侧延伸到SCN的中央亚核（SCNce）。小鼠SCN中的模式相似。NeuN-IR不与加压素、胆囊收缩素或血管活性肠多肽IR共定位。在仓鼠SCN中，许多细胞同时含有钙结合蛋白和NeuN-IR。NeuN-IR细胞在SCN中的分布是独特的，特别是关于其通常在核长度上的横向位置。NeuN-IR细胞的分布与代表SCN组织的大多数模式或与广泛接受的细分术语不一致。NeuN最近被鉴定为Fox-3蛋白。其在SCN中的功能尚不清楚，也不清楚为什么大部分SCN细胞不含NeuN-IR。
The suprachiasmatic nucleus (SCN) has several structural characteristics and cell phenotypes shared across species. Here, we describe a novel feature of SCN anatomy that is seen in both hamster and mouse. Frozen sections through the SCN were obtained from fixed brains and stained for the presence of immunoreactivity to neuronal nuclear protein (NeuN-IR) using a mouse monoclonal antibody which is known to exclusively identify neurons. NeuN-IR did not identify all SCN neurons as medial NeuNIR neurons were generally not present. In the hamster, NeuN-IR cells are present rostrally, scattered in the dorsal half of the nucleus. More caudally, the NeuN-IR cells are largely, but not exclusively, scattered inside the lateral and dorsolateral border. At mid- to mid-caudal SCN levels, a dense group of NeuN-IR cells extends from the dorsolateral border ventromedially to encompass the central subnucleus of the SCN (SCNce). The pattern is similar in the mouse SCN. NeuN-IR does not co-localize with either vasopressin-, cholecystokinin- or vasoactive intestinal polypeptide-IR. In the hamster SCNce, numerous cells contain both calbindin- and NeuN-IR. The distribution of NeuN-IR cells in the SCN is unique, especially with regard to its generally lateral location through the length of the nucleus. The distribution of NeuN-IR cells is not consistent with most schemas representing SCN organization or with terminology referring to its widely accepted subdivisions. NeuN has recently been identified as Fox-3 protein. Its function in the SCN is not known, nor is it known why a large proportion of SCN cells do not contain NeuN-IR.
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影响因子：
3.3
作者：
Glass, JD;Watanabe, M;Rutishauser, U
通讯作者：
Rutishauser, U
FOX-3和PSF相互作用以激活神经细胞特异性替代剪接。
影响因子：
14.9
作者：
Kim KK;Kim YC;Adelstein RS;Kawamoto S
通讯作者：
Kawamoto S
影响因子：
25
作者：
Gooley, JJ;Lu, J;Saper, CB
通讯作者：
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影响因子：
4.8
作者：
Karatsoreos, Ilia N.;Butler, Matthew P.;Silver, Rae
通讯作者：
Silver, Rae
影响因子：
3.3
作者：
Lesauter, J;Kriegsfeld, LJ;Silver, R
通讯作者：
Silver, R