Suppression of Proinflammatory Cytokines and Mediators in LPS-Induced RAW 264.7 Macrophages by Stem Extract of Alternanthera sessilis via the Inhibition of the NF-κB Pathway.
Suppression of Proinflammatory Cytokines and Mediators in LPS-Induced RAW 264.7 Macrophages by Stem Extract of Alternanthera sessilis via the Inhibition of the NF-κB Pathway.
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DOI：
10.1155/2018/3430684
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发表时间：
2018
影响因子：
4.1
通讯作者：
Arulselvan P
中科院分区：
文献类型：
--
作者：
Muniandy K;Gothai S;Badran KMH;Suresh Kumar S;Esa NM;Arulselvan P
空心莲子草是一种可食多汁草本植物，在地球仪的许多地区被广泛用作中草药。炎症是先天免疫系统的自然过程，伴随着促炎介质水平的增加，例如一氧化氮（NO）和前列腺素（PGE 2）;细胞因子，例如白介素6（IL-6）、白介素1β（IL-1β）和肿瘤坏死因子α（TNFα）;以及包括诱导型一氧化氮合酶（iNOS）和环氧合酶-2（考克斯-2）在内的酶通过活化的B细胞（NF-κB）的核因子κ轻链增强子的活化和核转位抑制蛋白IκBα的磷酸化导致p65亚基的磷酸化。短时间内的炎症对其治疗效果至关重要。然而，长期的炎症可能是有害的，因为它与许多慢性疾病有关，如伤口愈合延迟，心血管疾病，关节炎和自身免疫性疾病。因此，抑制慢性炎症的方法已被广泛研究。与此相一致，在本研究中，我们试图研究作为抗炎作用特征的促炎细胞因子和介质的抑制活性，通过使用A.在脂多糖-（LPS-）刺激的RAW 264.7巨噬细胞系中，结果表明，该提取物通过抑制IκBα的降解，抑制NF-κB亚单位p65的核转位，抑制NO和PGE 2等促炎介质、IL-6、IL-1β和TNFα等细胞因子以及覆盖iNOS和考克斯-2的酶的产生，从而抑制炎症通路的激活。这些结果表明，黄芪茎提取物具有较好的抗氧化活性。无柄可能是改善炎症相关并发症的有效候选物。
Alternanthera sessilis, an edible succulent herb, has been widely used as herbal drug in many regions around the globe. Inflammation is a natural process of the innate immune system, accompanied with the increase in the level of proinflammatory mediators, for example, nitric oxide (NO) and prostaglandin (PGE2); cytokines such as interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor alpha (TNFα); and enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) via the activation and nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) subunit p65 due to the phosphorylation of inhibitory protein, IκBα. Inflammation over a short period of time is essential for its therapeutic effect. However, prolonged inflammation can be detrimental as it is related to many chronic diseases such as delayed wound healing, cardiovascular disease, arthritis, and autoimmune disorders. Therefore, ways to curb chronic inflammation have been extensively investigated. In line with that, in this present study, we attempted to study the suppression activity of the proinflammatory cytokines and mediators as a characteristic of anti-inflammatory action, by using stem extract of A. sessilis in the lipopolysaccharide- (LPS-) stimulated RAW 264.7 macrophage cell line. The results showed that the extract has significantly inhibited the production of the proinflammatory mediators including NO and PGE2; cytokines comprising IL-6, IL-1β, and TNFα; and enzymes covering the iNOS and COX-2 by preventing the IκBα from being degraded, to inhibit the nuclear translocation of NF-κB subunit p65 in order to hinder the inflammatory pathway activation. These results indicated that the stem extract of A. sessilis could be an effective candidate for ameliorating inflammatory-associated complications.
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影响因子：
0.7
作者：
Fard MT;Arulselvan P;Karthivashan G;Adam SK;Fakurazi S
通讯作者：
Fakurazi S
影响因子：
--
作者：
Abdel-Hameed el-SS;Bazaid SA;Salman MS
通讯作者：
Salman MS
影响因子：
--
作者：
Arulselvan P;Fard MT;Tan WS;Gothai S;Fakurazi S;Norhaizan ME;Kumar SS
通讯作者：
Kumar SS
影响因子：
5.1
作者：
Hou, X. L.;Tong, Q.;Fang, J. G.
通讯作者：
Fang, J. G.
Lycorine 通过抑制 P38 和 STAT 激活来抑制 RAW264.7 细胞中脂多糖诱导的 iNOS 和 COX-2 上调，并提高 LPS 攻击后小鼠的存活率
影响因子：
5.6
作者：
Kang, Jingjing;Zhang, Yushun;Yin, Zhimin
通讯作者：
Yin, Zhimin