Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats
Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats
复制标题
乙醇胺通过改变断奶大鼠的肠道微生物组和粘膜抗应激能力来增强肠道功能
DOI：
10.1017/s0007114518001101
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发表时间：
2018-05
影响因子：
3.6
通讯作者：
Yin Yu Long
中科院分区：
文献类型：
--
作者：
Zhou Jian;Xiong Xia;Wang Ke Xing;Zou Li Jun;Ji Peng;Yin Yu Long
摘要牛奶中含有的乙醇胺（Etn）是磷脂酰乙醇胺的基本成分，是肠道上皮细胞和细菌增殖所必需的，对维持肠道微生物组和肠道发育至关重要。本研究使用21日龄Sprague-Dawley大鼠，在断奶后立即用0、250、500和1000 μm Etn饮水处理2周，研究Etn对肠道功能和微生物组的影响。对生长性能、肠道形态、抗氧化能力和粘膜免疫力以及肠道微生物群组成进行了评价。还进行了基于16 S RNA测序的宏基因组预测和代谢表型分析，以评估代谢功能的变化。结果表明，与对照组相比，500 μ mEtn组大鼠空肠超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）水平显著升高（P<0.05）。与对照组相比，不同水平的Etn改变了优势微生物，包括拟杆菌，变形菌，Elusimicrobia和Tenericutes。500 µm的Etn浓度改变了结肠微生物的代谢功能，有利于脂质和糖相关的代谢和生物合成。Etn还改变了代谢表型，如厌氧微生物计数和超过250 µm的氧化应激耐受性。这是关于Etn在改变肠道微生物群和肠道功能中的作用的第一份报告。我们的研究结果强调了Etn在塑造肠道微生物群落和促进肠道功能方面的重要作用，这可能为母乳喂养对婴儿肠道健康提供更好的见解。
Abstract Ethanolamine (Etn) contained in milk is the base constituent of phosphatidylethanolamine and is required for the proliferation of intestinal epithelial cells and bacteria, which is important for maintenance of the gut microbiome and intestinal development. The present study investigated the effect of Etn on intestinal function and microbiome using 21-d-old Sprague–Dawley rats treated with 0, 250, 500 and 1000 μm Etn in drinking water for 2 weeks immediately after weaning. Growth performance, intestinal morphology, antioxidant capacity and mucosal immunity, as well as gut microbiota community composition, were evaluated. Metagenomic prediction and metabolic phenotype analysis based on 16S RNA sequencing were also carried out to assess changes in metabolic functions. We found that weaned rats administered 500 μm Etn enhanced mucosal antioxidant capacity, as evidenced by higher superoxide dismutase and glutathione peroxidase levels in the jejunum (P<0·05) compared with those in the control group. Predominant microbes including Bacteroidetes, Proteobacteria, Elusimicrobia and Tenericutes were altered by different levels of Etn compared with the control group. An Etn concentration of 500 µm shifted colonic microbial metabolic functions that are in favour of lipid- and sugar-related metabolism and biosynthesis. Etn also altered the metabolic phenotypes such as anaerobic microbial counts, and oxidative stress tolerance at over 250 µm. This is the first report for a role of Etn in modifying gut microbiota and intestinal functions. Our findings highlighted the important role of Etn in shaping gut microbial community and promotes intestinal functions, which may provide a better insight of breast-feeding to infant’s gut health.
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影响因子：
4.4
作者：
S. Srikumar;T. Fuchs
通讯作者：
S. Srikumar;T. Fuchs
断奶过渡期间商品仔猪肠道微生物群的成熟发展
影响因子：
5.2
作者：
Chen L;Xu Y;Chen X;Fang C;Zhao L;Chen F
通讯作者：
Chen F
影响因子：
5.1
作者：
Nawrocki KL;Wetzel D;Jones JB;Woods EC;McBride SM
通讯作者：
McBride SM
影响因子：
4.2
作者：
Lackeyram, Dale;Yang, Chengbo;Fan, Ming Z.
通讯作者：
Fan, Ming Z.
影响因子：
3.1
作者：
A. Sintsova;Sara N Smith;S. Subashchandrabose;H. Mobley
通讯作者：
A. Sintsova;Sara N Smith;S. Subashchandrabose;H. Mobley