The Maturing Development of Gut Microbiota in Commercial Piglets during the Weaning Transition.
The Maturing Development of Gut Microbiota in Commercial Piglets during the Weaning Transition.
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断奶过渡期间商品仔猪肠道微生物群的成熟发展
DOI：
10.3389/fmicb.2017.01688
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发表时间：
2017
影响因子：
5.2
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Chen L;Xu Y;Chen X;Fang C;Zhao L;Chen F
早期断奶仔猪由于断奶应激和肠道发育不成熟，易发生腹泻。令人信服的证据表明，肠道微生物群对宿主健康至关重要。然而，断奶过渡期仔猪肠道菌群的组成和演替规律尚不清楚。在我们的两个试验中，共17个商品仔猪在中国江西省的养猪场进行了研究。分别于断奶前10 d、断奶当天、断奶后10 d、断奶后21 d收集新鲜粪便。通过Illumina Miseq平台的16 S rRNA基因V3-V4区测序评估粪便细菌组成。结果表明，断奶后仔猪肠道菌群变化较快，断奶后10 d达到相对稳定水平。α多样性随着仔猪日龄的增加而显著增加。哺乳仔猪的微生物群主要以梭杆菌属、乳杆菌属、拟杆菌属、埃希氏菌/志贺氏菌属和巨球菌属为代表。与断奶后的主要代表菌属Clostridium sensu stricto、Roseburia、Paraprevotella、Clostridium XIVa和Blautia形成鲜明对比。总之，我们描绘了断奶过渡期间仔猪肠道微生物群的发展。这项研究有助于我们了解商业仔猪肠道微生物群的成熟发育。
Early weaned piglets are vulnerable to diarrhea because of weaning stress and immaturity of intestinal tract. Compelling evidence suggests that gut microbiota is vital to host health. However, it is not well understood on the composition and succession of piglet gut microbiota during the weaning transition. In our two trials, total 17 commercial piglets were studied in a pig farm in Jiangxi Province, China. Fresh feces were collected for four times (10 days before weaned, weaned day, 10 days after weaned, 21 days after weaned) by rectal massage. Fecal bacterial composition was assessed by 16S rRNA gene V3–V4 regions sequencing by Illumina Miseq platform. The results showed that the gut microbiota of piglets shifted quickly after weaned and reached relatively stable level in 10 days after weaned. The alpha diversity increased significantly with the age of piglets. The microbiota of suckling piglets was mainly represented by Fusobacterium, Lactobacillus, Bacteroides, Escherichia/Shigella, and Megasphaera. This pattern contrasted with that of Clostridium sensu stricto, Roseburia, Paraprevotella, Clostridium XIVa, and Blautia, which were major representative genera after weaned. In summary, we delineated the development of piglet gut microbiota during the weaning transition. This study helps us understand the maturing development of gut microbiota in commercial piglets.
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影响因子：
5.4
作者：
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通讯作者：
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作者：
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