Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis.
Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis.
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DOI：
10.1016/j.vaccine.2007.01.055
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发表时间：
2007-05-04
期刊：
影响因子：
5.5
通讯作者：
Igimi S
中科院分区：
文献类型：
--
作者：
Kajikawa A;Satoh E;Leer RJ;Yamamoto S;Igimi S
构建了表达肠道沙门氏菌肠道血清型鞭毛抗原的重组干酪乳杆菌，并作为粘膜疫苗进行了评价。重组菌株的胃内免疫在小鼠中赋予针对沙门氏菌感染的保护性免疫。这种免疫在粪便或血清中均不产生抗原特异性抗体，但在离体再刺激致敏淋巴细胞时诱导IFN-γ的释放。结果表明，表达鞭毛蛋白的L.酪蛋白主要归因于细胞介导的免疫应答。此外，还观察了含L.还观察到干酪素。
A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-γ on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed.
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DOI：
10.1073/pnas.0500098102
发表时间：
2005-02-22
影响因子：
11.1
作者：
Mohamadzadeh, M;Olson, S;Klaenhammer, TR
通讯作者：
Klaenhammer, TR
影响因子：
5.5
作者：
Reveneau, N;Geoffroy, MC;Mercenier, A
通讯作者：
Mercenier, A
影响因子：
9.4
作者：
IBRAHIM, GF;FLEET, GH;WALKER, RA
通讯作者：
WALKER, RA
影响因子：
4.4
作者：
Peters, C;Peng, XH;Paterson, Y
通讯作者：
Paterson, Y
影响因子：
4.4
作者：
McSorley, SJ;Cookson, BT;Jenkins, MK
通讯作者：
Jenkins, MK