Upregulation of mitochondrial gene expression in PBMC from convalescent SARS patients.
Upregulation of mitochondrial gene expression in PBMC from convalescent SARS patients.
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DOI：
10.1007/s10875-006-9046-y
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发表时间：
2006-11
影响因子：
9.1
通讯作者：
Xu A
中科院分区：
文献类型：
--
作者：
Shao H;Lan D;Duan Z;Liu Z;Min J;Zhang L;Huang J;Su J;Chen S;Xu A
外周血单核细胞（PBMC）可被SARS- cov感染，外周血单核细胞（PBMC）可用于鉴定恢复期患者的基因表达谱和追踪宿主对SARS- cov感染的反应。本研究采用抑制减法杂交（suppression - subtract hybridization， SSH）方法研究了恢复期SARS患者PBMC中基因表达的变化。我们发现线粒体DNA （mtDNA）编码的基因明显上调，而线粒体现在被发现与抗病毒免疫密切相关。在SSH cDNA文库中鉴定出病毒基因M，表明SARS-CoV在体内长期存在。部分氧化应激敏感基因、热休克蛋白、转录因子、细胞因子均显著升高。电镜薄层观察显示PBMC溶酶体样颗粒增多，线粒体增多。这些结果为追踪宿主对SARS-CoV感染的反应提供了重要的细胞内线索，并提示线粒体在这一过程中的作用。
The observations that Lymphopenia is common in severe acute respiratory syndrome (SARS) patients and that peripheral blood mononuclear cell (PBMC) could be infected by SARS-CoV indicate that PBMC could be useful in identifying the gene expression profile in convalescent patients and tracing the host response to SARS-CoV infection. In this study, the altered genes expressions in the PBMC of convalescent SARS patients were investigated with suppression subtractive hybridization (SSH). We found that genes encoded by mitochondrial DNA (mtDNA) were obviously upregulated, while mitochondria were now found to be closely connected with antiviral immunity. The identification of a viral gene, M, in SSH cDNA library shows the long-term existence of SARS-CoV in vivo. In addition, some oxidative stress sensitive genes, heat shock proteins, transcription factors, and cytokines showed remarkable elevation. Thin-section electron microscope shows increased lysosome-like granule and mitochondria in PBMC of patients. These results provide important intracellular clue for tracing host response to SARS-CoV infection and suggest a role of mitochondria in that process.
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DOI：
10.1016/j.jcv.2004.12.019
发表时间：
2005-10
期刊：
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
影响因子：
--
作者：
Li Q;Wang L;Dong C;Che Y;Jiang L;Liu L;Zhao H;Liao Y;Sheng Y;Dong S;Ma S
通讯作者：
Ma S
影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
影响因子：
64.8
作者：
Meylan, E;Curran, J;Tschopp, R
通讯作者：
Tschopp, R
影响因子：
11.8
作者：
Miró, O;López, S;Cardellach, F
通讯作者：
Cardellach, F
影响因子：
30.5
作者：
Kawai, T;Takahashi, K;Akira, S
通讯作者：
Akira, S