Genomic Organization, Phylogenetic Comparison, and Differential Expression of the Nuclear Factor-Y Gene Family in Apple (Malus Domestica).
Genomic Organization, Phylogenetic Comparison, and Differential Expression of the Nuclear Factor-Y Gene Family in Apple (Malus Domestica).
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苹果核因子-Y 基因家族的基因组组织、系统发育比较和差异表达
DOI：
10.3390/plants10010016
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发表时间：
2020-12-24
期刊：
影响因子：
--
通讯作者：
Hou H
中科院分区：
文献类型：
--
作者：
Qu Y;Wang Y;Zhu J;Zhang Y;Hou H
核因子Y(NF-Y)作为一种转录因子，在植物的生长发育和逆境反应中起着重要的作用。然而，对苹果(Malus home tica Borkh.)核因子-Y家族的全基因组分析和功能研究还很少。到目前为止。在本研究中，我们通过序列比对分析，综合鉴定了苹果中43个MdNF-Y基因，包括11个MdNF-YA、22个MdNF-YBS和10个MdNF-YC，这些基因分散在三个亚群中。同一亚群中的成员具有相似的进化关系、基因结构和保守的模体。基因重复分析表明，所有基因都是分散的，其次是27个片段重复。此外，基于与8种植物的同源性分析，结果表明，在这些物种的进化过程中，一些同源基因被保存下来。顺式元件分析表明，MdNF-Ys在苹果生长发育过程中具有潜在的功能，并对非生物胁迫做出了响应。此外，在酵母双杂交实验中还研究了MdNF-Ys蛋白之间的相互作用。MdNF-Ys在组织特异性反应中的表达模式表现出差异性，可能在苹果的生长发育中发挥重要作用。随后，对5种非生物胁迫(ABA、干旱、高温、寒冷和盐度)进行了MdNF-Y基因全家族的RT-PCR，以确定它们的表达模式。综上所述，本研究将为进一步研究苹果生长发育和响应非生物胁迫的分子机制奠定基础。
The nuclear factor Y (NF-Y) as a transcription factor plays an important role in plants growth and development, and response to stress. However, few genome-wide analyzes and functional research of the NF-Y family has been undertaken in apple (Malus domestica Borkh.) so far. In this study, we comprehensively identified the 43 MdNF-Y genes in apple, which dispersedly distributed among the three subgroups based on their sequence alignment analysis, including 11 MdNF-YAs, 22 MdNF-YBs and 10 MdNF-YCs. The members in the same subgroups had similar evolution relationships, gene structures, and conserved motifs. The gene duplication analysis suggested that all the genes were dispersed followed by 27 segmental duplication. Moreover, based on synteny analysis of MdNF-Ys with eight plant species results suggested that some ortholog genes were preserved during the evolution of these species. Cis-element analysis showed potential functions of MdNF-Ys in apple growth and development and responded to abiotic stress. Furthermore, the interaction among MdNF-Ys protein were investigated in yeast two-hybrid assays. The expression patterns of MdNF-Ys in tissue-specific response reveled divergence and might play important role in apple growth and development. Subsequently, whole MdNF-Y genes family was carried out for RT-PCR in response to five abiotic stress (ABA, drought, heat, cold, and salinity) to identify their expression patterns. Taken together, our study will provide a foundation for the further study to the molecular mechanism of apple in growing development and response to abiotic stresses.
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