High-density integrated linkage map based on SSR markers in soybean.
High-density integrated linkage map based on SSR markers in soybean.
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DOI：
10.1093/dnares/dsp010
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发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
Ishimoto M
中科院分区：
文献类型：
--
作者：
Hwang TY;Sayama T;Takahashi M;Takada Y;Nakamoto Y;Funatsuki H;Hisano H;Sasamoto S;Sato S;Tabata S;Kono I;Hoshi M;Hanawa M;Yano C;Xia Z;Harada K;Kitamura K;Ishimoto M
一个饱和度高的分子连锁图谱是现代植物育种的先决条件。利用各种类型的分子标记已经为大豆开发了几个遗传图谱。简单序列重复（SSR）是具有高度等位变异的单位点标记，广泛适用于不同的基因型。我们已经在三个大豆重组自交群体中的一个或多个群体中定位了1810个SSR或序列标记位点（美国品种'Jack' ×日本品种'Fukuyutaka'，中国品种'Peking' ×日本品种'Akita'，和日本品种“Misuzudaizu”×中国育种系“Moshidou Gong 503”）并将这些标记与20个共有连锁群（LGs）进行了比对。该连锁图总长度为2442.9cM，标记数为70-114个，平均90.5个。我们研究了1238个SSR标记在23个大豆品种或品系和一个野生种质中的等位基因多样性。每个位点的等位基因数为2 ~ 7个，平均为2.8个。我们的高密度连锁图将有助于正在进行的和未来的基因组研究，如数量性状基因座分析和定位克隆，以及在大豆育种中的标记辅助选择。
A well-saturated molecular linkage map is a prerequisite for modern plant breeding. Several genetic maps have been developed for soybean with various types of molecular markers. Simple sequence repeats (SSRs) are single-locus markers with high allelic variation and are widely applicable to different genotypes. We have now mapped 1810 SSR or sequence-tagged site markers in one or more of three recombinant inbred populations of soybean (the US cultivar ‘Jack’ × the Japanese cultivar ‘Fukuyutaka’, the Chinese cultivar ‘Peking’ × the Japanese cultivar ‘Akita’, and the Japanese cultivar ‘Misuzudaizu’ × the Chinese breeding line ‘Moshidou Gong 503’) and have aligned these markers with the 20 consensus linkage groups (LGs). The total length of the integrated linkage map was 2442.9 cM, and the average number of molecular markers was 90.5 (range of 70–114) for the 20 LGs. We examined allelic diversity for 1238 of the SSR markers among 23 soybean cultivars or lines and a wild accession. The number of alleles per locus ranged from 2 to 7, with an average of 2.8. Our high-density linkage map should facilitate ongoing and future genomic research such as analysis of quantitative trait loci and positional cloning in addition to marker-assisted selection in soybean breeding.
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DOI：
10.1073/pnas.0402251101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Choi, HK;Mun, JH;Cook, DR
通讯作者：
Cook, DR
影响因子：
5.4
作者：
Diwan, N;Cregan, PB
通讯作者：
Cregan, PB
影响因子：
5.4
作者：
Cregan, PB;Mudge, J;Young, ND
通讯作者：
Young, ND
影响因子：
5.4
作者：
KEIM, P;BEAVIS, W;FREESTONE, R
通讯作者：
FREESTONE, R
影响因子：
4.1
作者：
Hossain, KG;Kawai, H;Harada, K
通讯作者：
Harada, K