Abscisic acid-responsive transcription factors PavDof2/6/15 mediate fruit softening in sweet cherry.
Abscisic acid-responsive transcription factors PavDof2/6/15 mediate fruit softening in sweet cherry.
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脱落酸反应转录因子PavDof2/6/15介导甜樱桃果实软化。
DOI：
10.1093/plphys/kiac440
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发表时间：
2022-11-28
期刊：
影响因子：
7.4
通讯作者：
Li, Tianhong
中科院分区：
文献类型：
--
作者：
Zhai, Zefeng;Xiao, Yuqin;Wang, Yanyan;Sun, Yueting;Peng, Xiang;Feng, Chen;Zhang, Xiang;Du, Bingyang;Zhou, Xin;Wang, Chao;Liu, Yang;Li, Tianhong
软化是果实成熟过程中的关键步骤，受多种植物激素的相互作用调节。脱落酸（ABA）和生长素的拮抗作用决定了果实的成熟和软化速度。然而，整合ABA和生长素信号调控果实软化的转录因子尚未确定。在这项研究中，我们通过对两个果实硬度不同的甜樱桃品种的转录组分析，鉴定了aba促进果实软化所必需的几个dna结合的One Finger （Dof）转录因子。我们发现，PavDof6直接结合编码细胞壁修饰酶的基因启动子来激活它们的转录，而PavDof2/15直接抑制它们的转录。甜樱桃果实中瞬时过表达PavDof6和PavDof2/15分别导致早熟和延迟软化。此外，我们发现生长素反应因子PavARF8，其编码基因的表达被ABA抑制，激活PavDof2/15的转录。此外，PavDof2/6/15和PavARF8直接结合9-顺式环氧类胡萝卜素双加氧酶1 （PavNCED1）启动子并调控其表达，形成aba介导的果实软化反馈机制。这些发现揭示了果实软化的生理框架，并建立了ABA-PavARF8-PavDofs模块与细胞壁修饰基因在介导果实软化中的直接功能联系。转录组分析鉴定了一组对脱落酸促进甜樱桃果实软化至关重要的转录因子。
Softening is a key step during fruit ripening that is modulated by the interplay between multiple phytohormones. The antagonistic action of abscisic acid (ABA) and auxin determines the rate of fruit ripening and softening. However, the transcription factors that integrate ABA and auxin signals to regulate fruit softening remain to be determined. In this study, we identified several DNA-binding with One Finger (Dof) transcription factors essential for ABA-promoted fruit softening, based on transcriptome analysis of two sweet cherry (Prunus avium L.) varieties with different fruit firmness. We show that PavDof6 directly binds to the promoters of genes encoding cell wall-modifying enzymes to activate their transcription, while PavDof2/15 directly repress their transcription. Transient overexpression of PavDof6 and PavDof2/15 in sweet cherry fruits resulted in precocious and delayed softening, respectively. In addition, we show that the auxin response factor PavARF8, the expression of whose encoding gene is repressed by ABA, activates PavDof2/15 transcription. Furthermore, PavDof2/6/15 and PavARF8 directly bind to the 9-cis-epoxycarotenoid dioxygenase 1 (PavNCED1) promoter and regulate its expression, forming a feedback mechanism for ABA-mediated fruit softening. These findings unveil the physiological framework of fruit softening and establish a direct functional link between the ABA–PavARF8–PavDofs module and cell-wall-modifying genes in mediating fruit softening. Transcriptome analysis identified a set of transcription factors essential for abscisic acid-promoted fruit softening in sweet cherry.
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