Genome-wide characterization of the WAK gene family and expression analysis under plant hormone treatment in cotton.
Genome-wide characterization of the WAK gene family and expression analysis under plant hormone treatment in cotton.
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棉花WAK基因家族的全基因组表征及植物激素处理下的表达分析
DOI：
10.1186/s12864-021-07378-8
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发表时间：
2021-01-28
期刊：
影响因子：
4.4
通讯作者：
Xiao G
中科院分区：
文献类型：
--
作者：
Dou L;Li Z;Shen Q;Shi H;Li H;Wang W;Zou C;Shang H;Li H;Xiao G
细胞壁相关激酶（WAK）是一类受体样激酶（RLK），直接参与植物细胞壁与细胞质之间的连接和通讯。WAK基因在水稻等植物中高度保守，已被鉴定，但在棉花中的研究很少。在本研究中，我们确定了29 GhWAK基因在陆地棉。系统进化分析表明，棉花WAK蛋白可分为5个进化枝。同线性和Ka/Ks分析结果表明，GhWAK基因主要来源于全基因组重复（WGD），主要处于纯化选择中。转录组数据和实时荧光定量PCR结果表明，97%的GhWAK基因在棉纤维和胚珠中高表达。GUS染色结果表明，GhWAK 5和GhWAK 16在拟南芥叶毛中表达。14个GhWAK基因被发现具有推定的赤霉素（GA）的启动子区域，其中13个显着诱导的GA处理的反应元件。10个GhWAK基因含有生长素（IAA）响应元件，其中9个基因在生长素处理下表达量显著增加。我们对G.揭示了GhWAK基因在棉花纤维细胞发育中的潜在作用。我们的数据也为今后研究GhWAK基因的功能作用提供了有用的资源。在线版本包含补充材料，可通过10.1186/s12864-021-07378-8获得。
Wall-associated kinases (WAK), one of the receptor-like kinases (RLK), function directly in the connection and communication between the plant cell wall and the cytoplasm. WAK genes are highly conserved and have been identified in plants, such as rice, but there is little research on the WAK gene family in cotton. In the present study, we identified 29 GhWAK genes in Gossypium hirsutum. Phylogenetic analysis showed that cotton WAK proteins can be divided into five clades. The results of synteny and Ka/Ks analysis showed that the GhWAK genes mainly originated from whole genome duplication (WGD) and were then mainly under purifying selection. Transcriptome data and real-time PCR showed that 97% of GhWAK genes highly expressed in cotton fibers and ovules. β-glucuronidase (GUS) staining assays showed that GhWAK5 and GhWAK16 expressed in Arabidopsis leaf trichomes. Fourteen GhWAK genes were found to possess putative gibberellin (GA) response elements in the promoter regions, 13 of which were significantly induced by GA treatment. Ten GhWAK genes contained auxin (IAA) response elements and the expression level of nine GhWAKs significantly increased under auxin treatment. We provide a preliminary analysis of the WAK gene family in G. hirsutum, which sheds light on the potantial roles of GhWAK genes in cotton fiber cell development. Our data also provides a useful resource for future studies on the functional roles of GhWAK genes. The online version contains supplementary material available at 10.1186/s12864-021-07378-8.
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