Oral inoculation of young dairy calves with Mycoplasma bovis results in colonization of tonsils, development of otitis media and local immunity.
Oral inoculation of young dairy calves with Mycoplasma bovis results in colonization of tonsils, development of otitis media and local immunity.
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DOI：
10.1371/journal.pone.0044523
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Simecka JW
中科院分区：
文献类型：
--
作者：
Maunsell F;Brown MB;Powe J;Ivey J;Woolard M;Love W;Simecka JW
因为M.牛中耳炎是一个经济上重要的问题，有必要了解疾病的发病机理，不仅要提高我们对导致这种疾病发展的因素的理解，而且要为改进的诊断测试和治疗的发展提供信息。口服M.受牛污染的牛奶与中耳炎的发生有关，但尚未得到明确证实。在目前的研究中，我们证明了口服M。牛感染的初乳可导致上行感染和中耳炎的发展。重要的是，M。发现牛具有先前未认识到的小牛扁桃体定殖的趋势，这很可能导致随后的中耳炎的发展。与此相反，经气管接种未能产生临床上显著的上呼吸道疾病，虽然诱导下呼吸道疾病。上呼吸道是M.经口接种小牛的牛特异性B细胞和粘膜伊加应答。本文介绍的口服接种感染途径特别适合研究扁桃体初始定植、感染扩大以及传播至下呼吸道和中耳期间宿主与病原体的相互作用。此外，它可用于研究潜在的新的预防或控制策略，特别是那些旨在限制扁桃体定植和/或传播到中耳的策略。
Because M. bovis otitis media is an economically important problem, there is a need to understand the pathogenesis of disease, not only to improve our understanding of the factors contributing to the development of this disease but also to inform the development of improved diagnostic tests and therapy. Oral ingestion of M. bovis-contaminated milk is linked, but not definitively proven, to development of otitis media. In the current study, we demonstrate that oral ingestion of M. bovis infected colostrum can result in an ascending infection and development of otitis media. Importantly, M. bovis was found to have a previously unrecognized tendency for colonization of the tonsils of calves, which most likely contributed to the subsequent development of otitis media. In contrast, transtracheal inoculation failed to produce clinically significant upper respiratory tract disease, although did induce lower respiratory tract disease. The upper respiratory tract was the major site of M. bovis-specific B cell and mucosal IgA responses in calves inoculated by the oral route. The oral inoculation route of infection presented here is particularly suited to the study of host-pathogen interactions during initial colonization of the tonsils, expansion of infection and dissemination to the lower respiratory tract and middle ear. In addition, it could be used to investigate potential new preventative or control strategies, especially those aimed at limiting colonization of the tonsils and/or spread to the middle ear.
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影响因子：
0.8
作者：
Maeda, T;Shibahara, T;Kadota, K
通讯作者：
Kadota, K
影响因子：
2.2
作者：
Ayling, RD;Bashiruddin, SE;Nicholas, RAJ
通讯作者：
Nicholas, RAJ
影响因子：
2.4
作者：
HOWARD, CJ;GOURLAY, RN;TAYLOR, G
通讯作者：
TAYLOR, G
影响因子：
3.1
作者：
Cartner, SC;Lindsey, JR;Simecka, JW
通讯作者：
Simecka, JW
影响因子：
4.4
作者：
Jones, HP;Tabor, L;Simecka, JW
通讯作者：
Simecka, JW