Complete sequences of 3' end coding region for structural protein genes of turkey coronavirus.
Complete sequences of 3' end coding region for structural protein genes of turkey coronavirus.
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DOI：
10.1016/j.virusres.2004.06.003
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发表时间：
2004-11
期刊：
影响因子：
5
通讯作者：
Wu CC
中科院分区：
文献类型：
--
作者：
Lin TL;Loa CC;Wu CC
利用RT-PCR技术扩增了火鸡冠状病毒（turkey coronavirus，TCoV）刺突蛋白（spike，S）基因5′端与核衣壳蛋白（nucleocapsid，N）基因3′端的6963个核苷酸的重叠片段。引物来源于传染性支气管炎病毒（IBV）的相应序列。对PCR产物进行克隆和测序，并分析其核酸结构及其与其他冠状病毒已发表序列的相似性。测序及序列分析显示，该基因有9个开放阅读框（ORF），按5′-3′顺序依次代表S蛋白基因、三顺反子基因3、膜蛋白基因、双顺反子基因5和N蛋白基因。TCoV的这些编码区的总体核酸结构与IBV的同源区非常相似。IBV的转录调控序列CT（T/G）AACAA在TCoV基因组中具有高度保守性。将基因3、M基因、基因5和N基因序列与IBV的相应序列进行配对比较，发现它们具有较高的相似性（82.1-92.0%）。基于推导的S、M或N蛋白的氨基酸序列的系统发育分析表明，TCoV与IBV毒株在相同的基因组谱系内聚类，而所有其他哺乳动物冠状病毒被分组为对应于抗原组I或II的单独簇。TCoV和IBV的S蛋白序列有很大差异，相似性仅为33.8-33.9%。
Overlapping fragments of genomic RNA spanning 6963 nucleotides from 5′ end of spike (S) protein gene to 3′ end of nucleocapsid (N) protein gene of turkey coronavirus (TCoV) were amplified by reverse-transcription-polymerase chain reaction (RT-PCR). The primers were derived from the corresponding sequences of infectious bronchitis virus (IBV). The PCR products were cloned and sequenced and their nucleic acid structure and similarity to published sequences of other coronaviruses were analyzed. Sequencing and subsequent analysis revealed 9 open reading frames (ORFs) representing the entire S protein gene, tricistronic gene 3, membrane (M) protein gene, bicistronic gene 5, and N protein gene in the order of 5′–3′. The overall nucleic acid structures of these encoding regions of TCoV were very similar to the homologous regions of IBV. The consensus transcription-regulating sequence (TRS) of IBV, CT(T/G)AACAA, was highly conserved in TCoV genome at the levels of nucleotide sequence and location in regarding to the initiation codon of individual genes. Pair-wise comparison of gene 3, M gene, gene 5, or N gene sequences with their counterparts of IBV revealed high levels (82.1–92.0%) of similarity. Phylogenetic analysis based on the deduced amino acid sequences of S, M, or N protein demonstrated that TCoV was clustered within the same genomic lineage as the IBV strains while all the other mammalian coronaviruses were grouped into separate clusters corresponding to antigenic groups I or II. There were substantial differences of S protein sequence between TCoV and IBV with only 33.8–33.9% of similarity.
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影响因子：
2.7
作者：
González JM;Gomez-Puertas P;Cavanagh D;Gorbalenya AE;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
3.7
作者：
HEROLD, J;RAABE, T;SIDDELL, SG
通讯作者：
SIDDELL, SG
影响因子：
3.1
作者：
Akin, A;Wu, CC;Lin, TL
通讯作者：
Lin, TL
影响因子：
2.8
作者：
Cavanagh, D;Mawditt, K;Gough, RE
通讯作者：
Gough, RE
DOI：
10.1073/pnas.91.6.2216
发表时间：
1994-03-15
影响因子：
11.1
作者：
BARNES, WM
通讯作者：
BARNES, WM