Synthesis of ABC-triblock peptide-polymer conjugates for the positioning of peptide segments within block copolymer aggregates
Synthesis of ABC-triblock peptide-polymer conjugates for the positioning of peptide segments within block copolymer aggregates
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ABC-三嵌段肽-聚合物缀合物的合成，用于将肽片段定位在嵌段共聚物聚集体中
DOI：
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
H. Börner
中科院分区：
文献类型：
--
作者：
M. G. J. T. Cate;H. Börner
介绍了由中心寡肽段和末端亲水性聚环氧乙烷（PEO）以及疏水性聚丙烯酸丁酯（PBA）嵌段组成的两亲性abc -三嵌段肽-聚合物偶联物的合成。在水溶液中，这些功能偶联物的两亲性片段引导自组装成嵌段共聚物胶束，而功能寡肽则定位于亲疏水界面。为此，采用固相支撑肽合成技术（SPPS）合成了PEO-Arg - 10共轭物。负载的前体缀合物在肽段的氨基端被链转移部分功能化，用于RAFT聚合。随后，将AB宏观cta从载体中分离出来，用于在溶液中均匀聚合BA。RAFT自由基聚合过程可以控制pba -嵌段的分子量，从而产生具有低多分散性的两亲性abc -偶联物。光散射和原子力显微镜显示，三嵌段肽-聚合物共轭物在水中组装成胶束聚集体。abc -三嵌段共轭物的分子结构控制了聚合体中功能性但昂贵的寡肽片段的位置，而廉价的合成聚合物嵌段则决定了聚合行为。考虑到共轭物的设计，中心Arg - 10片段可以定位在疏水和亲水聚合物块之间的界面上，从而形成具有精确可控功能的功能域。
The synthesis of amphiphilic ABC-triblock peptide-polymer conjugates comprising a central oligopeptide segment and terminal hydrophilic poly(ethylene oxide) (PEO) as well as hydrophobic poly(butyl acrylate) (PBA) blocks is described. In aqueous solution, the amphiphilic segments of these functional conjugates direct the self-assembly into block copolymer micelles, while the functional oligopeptide is positioned at the hydrophilic and hydrophobic interface. For that the conjugate PEO-Arg 10 was synthesized using solid-phase supported peptide synthesis techniques (SPPS). The supported precursor conjugate was functionalized at the amino-terminus of the peptide segment with a chain-transfer moiety for RAFT polymerization. Subsequently, the AB macro-CTA was liberated from the support and used to polymerize BA homogenously in solution. The RAFT radical polymerization process allowed controlling the molecular weight of the PBA-block, leading to amphiphilic ABC-conjugates with low polydispersities. The triblock peptide-polymer conjugate assembles in water into micellar aggregates, as was shown by light scattering and AFM. The molecular architecture of the ABC-triblock conjugate controls the positioning of the functional but expensive oligopeptide-segment within the aggregates, while the inexpensive synthetic polymer blocks are determining the aggregation behavior. Considering the design of the conjugate, the central Arg 10 segment can be expected to be positioned at the interface between the hydrophobic and the hydrophilic polymer blocks, resulting in the formation of a functional domain with precisely controllable functionalities.
影响因子：
10.8
作者：
Nasongkla, Norased;Bey, Erik;Gao, Jinming
通讯作者：
Gao, Jinming
影响因子：
6.2
作者：
Becker, ML;Liu, JQ;Wooley, KL
通讯作者：
Wooley, KL
影响因子：
6.2
作者：
Irvine, DJ;Mayes, AM;Griffith, LG
通讯作者：
Griffith, LG