Genome characterization of feline morbillivirus from Italy.

Genome characterization of feline morbillivirus from Italy.
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DOI:
10.1016/j.jviromet.2016.05.002
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发表时间:
2016-08
影响因子:
3.1
通讯作者:
Lorusso A
Lorusso A
中科院分区:
医学4区
文献类型:
--
作者:
Marcacci M;De Luca E;Zaccaria G;Di Tommaso M;Mangone I;Aste G;Savini G;Boari A;Lorusso A

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猫麻疹病毒(FeMV)Piuma/2015的全基因组序列已通过SISPA和NGS的组合从患有慢性肾病的猫的感染尿液样本中获得。与来自香港的早期FeMV检测到最高的序列同一性。欧洲FeMV内存在序列异质性,德国和土耳其的FeMV存在与Piuma/2015不同。最近在意大利的一只肾病猫的尿样中通过RT-PCR鉴定出猫麻疹病毒(FeMV)。在这份报告中,我们描述了从感染尿样纯化的RNA开始,通过序列独立单引物扩增方法(SISPA)和下一代测序(NGS)的组合获得的菌株Piuma/2015的全基因组序列。德国和土耳其存在与Piuma/2015不同的猫FeMV,表明欧洲存在FeMV异质性,正如之前在日本和中国所述。
The whole genome sequence of feline morbillivirus (FeMV) Piuma/2015 has been obtained by the combination of SISPA and NGS starting from the infected urine sample of a cat suffering of chronic kidney disease. The highest sequence identity was detected with early FeMVs from Hong Kong. Sequence heterogeneity exists within European FeMVs as for the existence of FeMVs in Germany and Turkey divergent from Piuma/2015. Feline morbillivirus (FeMV) has been recently identified by RT-PCR in the urine sample of a nephropathic cat in Italy. In this report, we describe the whole genome sequence of strain Piuma/2015 obtained by combination of sequence independent single primer amplification method (SISPA) and next generation sequencing (NGS) starting from RNA purified from the infected urine sample. The existence in Germany and Turkey of FeMVs from cats divergent from Piuma/2015, suggests the presence of FeMV heterogeneity in Europe as it has been described previously in Japan and China.
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