Metagenomic and metabolomic analyses show correlations between intestinal microbiome diversity and microbiome metabolites in ob/ob and ApoE(-/-) mice.
Metagenomic and metabolomic analyses show correlations between intestinal microbiome diversity and microbiome metabolites in ob/ob and ApoE(-/-) mice.
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宏基因组学和代谢组学分析显示 ob/ob 和 ApoE 小鼠肠道微生物组多样性与微生物组代谢物之间的相关性
DOI：
10.3389/fnut.2022.934294
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发表时间：
2022
影响因子：
5
通讯作者：
Wei, Hong
中科院分区：
文献类型：
--
作者：
Dong, Sashuang;Wu, Chengwei;He, Wencan;Zhong, Ruimin;Deng, Jing;Tao, Ye;Zha, Furong;Liao, Zhenlin;Fang, Xiang;Wei, Hong
肥胖和动脉粥样硬化是最常见的代谢性疾病。ApoE−/−和ob/ob小鼠被广泛用作研究这些疾病发病机制的模型。然而，肠道微生物、肠道噬菌体和代谢物在这两种疾病模型中如何变化尚不清楚。在这里，我们使用野生型C57 BL/6 J（Wt）小鼠作为正常对照，通过宏基因组学和代谢组学分析ob/ob和ApoE−/−小鼠的肠道古菌，细菌，噬菌体和微生物代谢产物。对肠道古菌的分析表明，与Wt和ApoE−/−组相比，ob/ob组中甲烷短杆菌和嗜盐菌的丰度分别显著增加和减少（p < 0.05）。与Wt组相比，ob/ob小鼠中肠杆菌属、Alistipes属、拟杆菌属、普雷沃氏菌属、Rikenella属、Barnesiella属、卟啉单胞菌属、里默氏菌属和双歧杆菌属的相对丰度显著降低（p < 0.05），ApoE−/−组中阿克曼氏菌属的相对丰度显著降低。A. muciniphila和L.与Wt组相比，ob/ob和ApoE−/−组中的鼠尾静脉分别显著减少和增加（p < 0.05）。Lactobacillus_ prophage_Lj 965和Lactobacillus _ prophage _Lj 771在ob/ob小鼠中的丰度显著高于Wt小鼠。对氨酰-tRNA生物合成代谢途径的分析显示，与ob/ob小鼠相比，ApoE−/−小鼠中富含的苯丙氨酸、谷氨酰胺、甘氨酸、丝氨酸、蛋氨酸、缬氨酸、丙氨酸、赖氨酸、异亮氨酸、亮氨酸、苏氨酸、色氨酸和酪氨酸的化合物下调。氨酰-tRNA合成酶被认为是代谢性疾病的表现，并且与肥胖、动脉粥样硬化和2型糖尿病密切相关。这些数据为这些疾病的可能原因提供了新的见解，并为研究代谢疾病模型中各种食物营养素的调节提供了基础。整体实验设计。
Obesity and atherosclerosis are the most prevalent metabolic diseases. ApoE−/− and ob/ob mice are widely used as models to study the pathogenesis of these diseases. However, how gut microbes, gut bacteriophages, and metabolites change in these two disease models is unclear. Here, we used wild-type C57BL/6J (Wt) mice as normal controls to analyze the intestinal archaea, bacteria, bacteriophages, and microbial metabolites of ob/ob and ApoE−/− mice through metagenomics and metabolomics. Analysis of the intestinal archaea showed that the abundances of Methanobrevibacter and Halolamina were significantly increased and decreased, respectively, in the ob/ob group compared with those in the Wt and ApoE−/− groups (p < 0.05). Compared with those of the Wt group, the relative abundances of the bacterial genera Enterorhabdus, Alistipes, Bacteroides, Prevotella, Rikenella, Barnesiella, Porphyromonas, Riemerella, and Bifidobacterium were significantly decreased (p < 0.05) in the ob/ob mice, and the relative abundance of Akkermansia was significantly decreased in the ApoE−/− group. The relative abundances of A. muciniphila and L. murinus were significantly decreased and increased, respectively, in the ob/ob and ApoE−/− groups compared with those of the Wt group (p < 0.05). Lactobacillus_ prophage_ Lj965 and Lactobacillus _ prophage _ Lj771 were significantly more abundant in the ob/ob mice than in the Wt mice. Analysis of the aminoacyl-tRNA biosynthesis metabolic pathway revealed that the enriched compounds of phenylalanine, glutamine, glycine, serine, methionine, valine, alanine, lysine, isoleucine, leucine, threonine, tryptophan, and tyrosine were downregulated in the ApoE−/− mice compared with those of the ob/ob mice. Aminoacyl-tRNA synthetases are considered manifestations of metabolic diseases and are closely associated with obesity, atherosclerosis, and type 2 diabetes. These data offer new insight regarding possible causes of these diseases and provide a foundation for studying the regulation of various food nutrients in metabolic disease models. Overall experimental design.
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影响因子：
32.4
作者：
Hezaveh K;Shinde RS;Klötgen A;Halaby MJ;Lamorte S;Ciudad MT;Quevedo R;Neufeld L;Liu ZQ;Jin R;Grünwald BT;Foerster EG;Chaharlangi D;Guo M;Makhijani P;Zhang X;Pugh TJ;Pinto DM;Co IL;McGuigan AP;Jang GH;Khokha R;Ohashi PS;O'Kane GM;Gallinger S;Navarre WW;Maughan H;Philpott DJ;Brooks DG;McGaha TL
通讯作者：
McGaha TL
影响因子：
9
作者：
通讯作者：
--
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
影响因子：
9.8
作者：
Ahmad, Ashfaq;Ali, Tahir;Kim, Myeong Ok
通讯作者：
Kim, Myeong Ok
影响因子：
16.2
作者：
Gao, Jian;Guo, Xiaoyu;Han, Tianshu
通讯作者：
Han, Tianshu