Polymerase chain reaction.

Polymerase chain reaction.
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DOI:
10.1038/jid.2013.1
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发表时间:
2013-03
影响因子:
6.5
通讯作者:
Avashia, Nidhi
Avashia, Nidhi
中科院分区:
医学1区
文献类型:
--
作者:
Garibyan, Lilit;Avashia, Nidhi

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聚合酶链反应(PCR)的出现从发现之日起就彻底改变了生物科学(Mullis,1990)。这是第一次,PCR允许特异性检测和大量DNA的生产。基于PCR的策略已经推动了巨大的科学努力,如人类基因组计划。该技术被临床医生和研究人员广泛用于诊断疾病,克隆和测序基因,并以快速和非常敏感的方式进行复杂的定量和基因组研究。经典PCR方法最重要的医学应用之一是病原体检测。此外,PCR检测在法医学中用于识别罪犯。由于其广泛的应用,重要的是要了解PCR的基本原理,以及如何修改其使用,以提供基因和基因组的复杂分析。
The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was discovered (Mullis, 1990). For the first time, PCR allowed for specific detection and production of large amounts of DNA. PCR-based strategies have propelled vast scientific endeavors such as the Human Genome Project. The technique is widely used by clinicians and researchers to diagnose diseases, clone and sequence genes, and carry out sophisticated quantitative and genomic studies in a rapid and very sensitive manner. One of the most important medical applications of the classic PCR method is detection of pathogens. In addition, the PCR assay is used in forensic medicine to identify criminals. Because of its widespread use, it is important to understand the basic principles of PCR and how its use can be modified to provide for sophisticated analysis of genes and the genome.
DOI: 10.1373/clinchem.2012.191445
发表时间: 2012-12-01
期刊: CLINICAL CHEMISTRY
影响因子: 9.3
作者:
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