Toggled RNA aptamers against aminoglycosides allowing facile detection of antibiotics using gold nanoparticle assays.
Toggled RNA aptamers against aminoglycosides allowing facile detection of antibiotics using gold nanoparticle assays.
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DOI：
10.1021/ac300815c
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发表时间：
2012-08-07
影响因子：
7.4
通讯作者：
Stockley, Peter G.
中科院分区：
文献类型：
--
作者：
Derbyshire, Nicola;White, Simon J.;Bunka, David H. J.;Song, Lei;Stead, Sara;Tarbin, Jonathan;Sharman, Matthew;Zhou, Dejian;Stockley, Peter G.
我们已经使用系统进化配体指数富集（SELEX）分离RNA适配体对氨基糖苷类抗生素。针对四对氨基糖苷类切换SELEX轮，目的是分离识别保守结构特征的试剂。所得适体以纳摩尔亲和力结合它们的选择靶标。它们也结合结构上不太相关的靶标，尽管它们对这类抗生素显示出明确的特异性。我们表明，这种缺乏氨基糖苷类特异性是一个共同的属性，以前选择对单一化合物的适体，并描述为“特定的”。广泛的目标特异性适体将是理想的传感器检测整个类别的氨基糖苷类。我们使用配体诱导的金纳米粒子的聚集与我们的适配体作为这些化合物的快速和灵敏的测定。与DNA适体相反，未修饰的RNA适体不能在该测定中用作识别配体，而2′-氟嘧啶衍生物可靠地起作用。我们讨论了这些试剂作为药物残留传感器的可能应用，以及SELEX结果强调的了解氨基糖苷类适体识别结构基础的挑战。
We have used systematic evolution of ligands by exponential enrichment (SELEX) to isolate RNA aptamers against aminoglycoside antibiotics. The SELEX rounds were toggled against four pairs of aminoglycosides with the goal of isolating reagents that recognize conserved structural features. The resulting aptamers bind both of their selection targets with nanomolar affinities. They also bind the less structurally related targets, although they show clear specificity for this class of antibiotics. We show that this lack of aminoglycoside specificity is a common property of aptamers previously selected against single compounds and described as “specific”. Broad target specificity aptamers would be ideal for sensors detecting the entire class of aminoglycosides. We have used ligand-induced aggregation of gold-nanoparticles coated with our aptamers as a rapid and sensitive assay for these compounds. In contrast to DNA aptamers, unmodified RNA aptamers cannot be used as the recognition ligand in this assay, whereas 2′-fluoro-pyrimidine derivatives work reliably. We discuss the possible application of these reagents as sensors for drug residues and the challenges for understanding the structural basis of aminoglycoside-aptamer recognition highlighted by the SELEX results.
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影响因子：
4.1
作者：
Patel, D J;Suri, A K
通讯作者：
Suri, A K
DOI：
10.1080/19440041003677483
发表时间：
2010-01-01
影响因子：
2.9
作者：
Gaudin, V.;Hedou, C.;Verdon, E.
通讯作者：
Verdon, E.
影响因子：
4.8
作者：
BEIGELMAN, L;MCSWIGGEN, JA;USMAN, N
通讯作者：
USMAN, N
影响因子：
--
作者：
Jiang, LC;Suri, AK;Patel, DJ
通讯作者：
Patel, DJ
影响因子：
12.6
作者：
Kim, Yeon Seok;Kim, Joong Hyun;Gu, Man Bock
通讯作者：
Gu, Man Bock