Fusobacterium nucleatum is associated with worse prognosis in Lauren's diffuse type gastric cancer patients.
Fusobacterium nucleatum is associated with worse prognosis in Lauren's diffuse type gastric cancer patients.
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DOI：
10.1038/s41598-020-73448-8
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发表时间：
2020-10-01
影响因子：
4.6
通讯作者：
Link A
中科院分区：
文献类型：
--
作者：
Boehm ET;Thon C;Kupcinskas J;Steponaitiene R;Skieceviciene J;Canbay A;Malfertheiner P;Link A
具核梭杆菌（Fusobacterium nucleatum, F.细胞核）在原发性结直肠癌（CRC）和匹配的转移中经常被检测到，并且与较差的预后相关。我们研究了F.胃癌细胞核与胃癌前病变的关系，以及胃癌细胞核对胃癌患者预后的潜在价值。梭杆菌属和F.使用基于探针的qPCR在来自胃肠道的各种样品中定量细胞核，所述样品包括配对的CRC和GC组织。梭杆菌属和F.细胞核在结直肠癌和胃癌肿瘤组织中比在非肿瘤组织中更常见。与GC患者相比，CRC患者的频率和细菌载量较高。F.细胞核阳性与慢性胃炎或癌前病变如肠上皮化生无关。F.在Lauren's弥漫型胃癌患者中，细胞核阳性与总生存率显著降低相关，而肠型胃癌患者则无此相关性.与性别、幽门螺杆菌感染状况、肿瘤分期或肿瘤部位无关。然而，F.细胞核与患者年龄呈正相关，且总体长散布元件-1 DNA甲基化有降低趋势。总之，本研究为F.结果表明，F.细胞核阳性者预后差。需要进一步研究以探索相关机制和靶向抗生素治疗GC患者的潜在治疗获益。
Fusobacterium nucleatum (F. nucleatum) is frequently detected in primary colorectal cancer (CRC) and matching metastasis, and has been linked to a worse prognosis. We investigated the presence of F. nucleatum in gastric cancer (GC) and gastric preneoplastic conditions of the stomach, and its potential prognostic value in GC patients. Fusobacterium spp. and F. nucleatum were quantified in various specimens from gastrointestinal tract including paired CRC and GC tissues using probe-based qPCR. Fusobacterium spp. and F. nucleatum were more frequently found in tumorous tissue of CRC and GC compared to non-tumorous tissues. The frequency and bacterial load were higher in CRC compared to GC patients. F. nucleatum positivity showed no association to chronic gastritis or preneoplastic conditions such as intestinal metaplasia. F. nucleatum-positivity was associated with significantly worse overall survival in patients with Lauren’s diffuse type, but not with intestinal type GC. There was no association with gender, Helicobacter pylori-status, tumor stage or tumor localization. However, F. nucleatum was positively associated with patient’s age and a trend for a lower global long interspersed element-1 DNA methylation. In conclusion, our work provides novel evidence for clinical relevance of F. nucleatum in GC by showing an association between F. nucleatum positivity with worse prognosis of patients with Laurens’s diffuse type gastric cancer. Further studies are necessary to explore related mechanistic insights and potential therapeutic benefit of targeted antibiotic treatment in GC patients.
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影响因子：
4.3
作者：
Link A;Kupcinskas J
通讯作者：
Kupcinskas J
影响因子：
4.6
作者：
Aviles-Jimenez F;Vazquez-Jimenez F;Medrano-Guzman R;Mantilla A;Torres J
通讯作者：
Torres J
影响因子：
4.6
作者：
Kasper, Stephen H.;Morell-Perez, Carolina;Hett, Erik C.
通讯作者：
Hett, Erik C.
影响因子：
10.1
作者：
Hamada T;Zhang X;Mima K;Bullman S;Sukawa Y;Nowak JA;Kosumi K;Masugi Y;Twombly TS;Cao Y;Song M;Liu L;da Silva A;Shi Y;Gu M;Li W;Koh H;Nosho K;Inamura K;Keum N;Wu K;Meyerhardt JA;Kostic AD;Huttenhower C;Garrett WS;Meyerson M;Giovannucci EL;Chan AT;Fuchs CS;Nishihara R;Giannakis M;Ogino S
通讯作者：
Ogino S
影响因子：
5.7
作者：
Abed J;Maalouf N;Parhi L;Chaushu S;Mandelboim O;Bachrach G
通讯作者：
Bachrach G