Evaporative Heat Moisture Control in Solid Substrate Fermentation of Wheat Straw for Cellulase Production

摘要本研究的目的是开发一套自动化、电脑化和一体化的蒸发热-湿控制系统，用于利用里氏木霉对小麦秸秆进行氨冻胀发酵生产纤维素酶。该系统是基于吹干空气通过潮湿的基材，使控制蒸发冷却，同时通过冷水喷雾补充失去的水。固体底物发酵（SSF）可以替代深层发酵（SF），从小麦秸秆和玉米秸秆等农业残留物中生产燃料和动物饲料。与SF相比，SSF的主要优点是基于基材和产品的低含水量的高体积生产率。这减少了发酵罐体积，简化了技术，提高了产品浓度，节省了除水成本。SSF商业化最关键的问题是湿度和温度的控制。pi计划设计和测试一个完全自动化和计算机化的系统，该系统基于将干燥的空气吹过潮湿的基材，以控制蒸发热量的排出，同时通过冷水喷雾补充失去的水分，以保持恒定的水分。如果这个概念可以在这项研究中得到证实，将为SSF过程的扩大提供一个现实的技术基础。

The objective of this research is to develop an automated, computerized and integrated evaporative heat-moisture control system in the solid substrate fermentation of ammonia freeze explosion treated wheat straw by Trichoderma reesei for cellulase production. The system is based on blowing dry air through the moist substrate to cause controlled evaporative cooling, at the same time replenishing lost water by cool water spray. Solid substrate fermentation (SSF) could be an alternative to submerged fermentation (SF) for producing fuel and animal feed from agricultural residues such as wheat straw and corn stover. The main advantage of SSF, compared to SF, is the high volumetric productivity which is based on the low water content of substrate and product. This reduces fermenter volume, simplifies technology, increases product concentration and saves the cost of water removal. The most critical problem for SSF commercialization is the control of moisture and temperature. The PIs plan to design and test a completely automated and computerized system based on blowing dry air through the moist substrate to cause controlled evaporative heat removal, and at the same time replenishing lost water by cool water spray to maintain constant moisture. If the concept can be proven in this research, a realistic technical base will be available for the scale-up of SSF processes.