U.S.-Norway Cooperative Research: Compartmental Modeling ofthe Metabolism of Retinol and Retinol-Binding Protein

该奖项将使迈克尔·H博士。绿色合作伙伴 Kaare R.诺鲁姆、卢恩·布洛姆霍夫和特隆德贝格， 挪威，奥斯陆。 他们将研究维生素A的代谢， 运用计算机辅助的、基于模型的 对以下数据进行房室分析：（1） 维生素A中的视黄醇和视黄醇结合蛋白（RBP）-充足 大鼠，（2）细胞内转运和分泌的动力学 维生素A缺乏大鼠的视黄醇，和（3）动力学 受体介导的视黄醇/RBP的内吞作用， 星状大鼠肝细胞。 他们的研究将有助于发展和量化 生理学相关的房室模型，其描述了 全身和肝脏视黄醇和RBP代谢的动力学。 他们将获得以下方面的信息：（1） 血浆、肝脏和肝外的RBP与视黄醇再循环 组织，（2）受影响的肝脏调节途径 维生素A缺乏症;（3）细胞内和亚细胞内 体外肝细胞中视黄醇和RBP的转运。 的 这些实验是他们的延续和延伸。 以往成功的研究合作。 绿色博士是一位 维生素A分析方法专家，基于模型 房室分析和全身维生素A代谢。 的 挪威研究人员在受体介导的 内吞作用、肝细胞分离和培养以及维生素A 代谢和方法。

This award will enable Dr. Michael H. Green to collaborate with Drs. Kaare R. Norum, Rune Blomhoff, and Trond Berg, University of Oslo, Norway. They will study the metabolism of vitamin A and will apply the unique approach of computer-assisted, model-based compartmental analysis to data on (1) the in vivo turnover of retinol and retinol-binding protein (RBP) in vitamin A - sufficient rats, (2) the dynamics of intracellular transport and secretion of retinol in vitamin A - deficient rats, and (3) the kinetics of receptor-mediated endocytosis of retinol/RBP in parenchymal and stellate rat liver cells. Their studies will facilitate the development and quantification of physiologically relevant compartmental models which describe the dynamics of whole body and hepatic retinol and RBP metabolism. They will gain information on (1) the nature and extent of RBP vs. retinol recycling among plasma, liver, and extrahepatic tissues, (2) the hepatic regulatory pathways which are affected by vitamin A deficiency, and (3) the intracellular and subcellular transport of retinol and RBP in hepatic cells in vitro. The proposed experiments are a continuation and extension of their previous successful research collaboration. Dr. Green is an expert in analytical methods for vitamin A, model-based compartmental analysis, and whole body vitamin A metabolism. The Norwegian researchers are specially skilled in receptor-mediated endocytosis, liver cell separation and culture, and vitamin A metabolism and methods.