Development of an Advanced Laboratory Course in Biochemical Techniques and Instrumentation

该项目将导致本科生物化学实验课程的发展，强调现代技术和仪器。实验课程将补充正在进行的生物化学讲座课程，并将大大加强北卡罗莱纳大学威尔明顿分校学生的生物化学教育。该项目将提供一套仪器（包括自动记录滴定系统、液相色谱系统、密度计、制备超离心机和电泳装置），主要用于新的生物化学实验课程，也用于物理和分析化学课程。学生将获得缓冲液和其他试剂制备、蛋白质分析、蛋白质纯化、蛋白质和DNA电泳、酶动力学、胰蛋白酶消化、膜泡构建、DNA分离和放射性同位素标记等方面的经验。实验工作将阐明生物化学中的重要概念，并将帮助学生熟练掌握广泛使用的生物化学技术。

This project will result in the development of an undergraduate biochemistry laboratory course that emphasizes modern techniques and instrumentation. The laboratory course will complement the ongoing biochemistry lecture course, and will enhance substantially the biochemistry education of students at the University of North Carolina at Wilmington. This project will provide a suite of instruments (including an automatic recording titration system, a liquid chromatography system, a densitometer, a preparative ultracentrifuge, and an electrophoresis unit) to be used primarily in the new biochemistry laboratory course and also in physical and analytical chemistry courses. Students will gain experience with buffer and other reagent preparation, protein assays, protein purification, protein and DNA electrophoresis, enzyme kinetics, tryptic peptide digestion, membrane vesicle construction, DNA isolation, and radioisotope labelling. The experimental work will illustrate important concepts in biochemistry, and will help students attain proficiency in widely used biochemical techniques.