Presidential Young Investigator Award: Protein Expression in E. coli During Transitional Growth.

旁白：该奖项支持一位总统青年研究员（PYI）研究各种生化工程问题。目前的工作主要集中在细胞过程，酶学，以及为植物设计开发分析和评估框架。已完成的工作包括生理功能（如信息转导）相关途径特性在结构（活性/调节机制组织）和操作点（代谢物状态载体）方面的权衡论证，以及单活性和多活性酶膜反应器系统的基础研究。未来的工作包括对细胞生长动力学、调节和能量学中起作用的潜在分子和物理过程的实验和理论研究。将设计新型生物反应器来研究物理、化学和生物现象，以便更好地了解反应器的操作。生物过程设计，如开发框架工程，从而对关键设计的影响，可操作的过程（细胞，分子）可以进行评估。此外，还将研究蛋白质转运现象，以确定构象状态和自结合程度的改变如何影响进入/在多孔分离介质中的迁移性。

Narravtive: This award supports a Presidential Young Investigator (PYI) to study a variety of Biochemical Engineering problems. Current work focuses on cellular processes, enzymology, and developing analysis and evaluation frameworks for plant design. Work that has been completed includes a demonstration that physiological function (e.g. information transduction) linked pathway properties trade off with respect to structure (activity/regulatory mechanism organization) and operating point (metabolite state vector), and fundamental investigations with single and multiactivity enzyme-membrane reactor systems. Future work includes experimental and theoretical studies of underlying molecular and physical processes that play a role in growth kinetics, regulation, and energetics of cells. Novel bioreactors will be devised to study physical, chemical and biological phenomena for a better understanding of reactor operation. Bioprocess design, such as developing frame works whereby the impact on design of key, manipulable processes (cellular, molecular) can be assessed. Also, protein transport phenomena will be investigated to determine how alteration of conformational state and extent of self association influences mobility into/within porous separation media.