Genetic Hypervariability in Halobacterium Halobium

极端嗜盐古细菌，盐生盐杆菌， 有能力合成细胞内充满气体的 囊泡浮力调节光的可用性， 氧气 气体囊泡合成（Vac）的突变率是 在H. 盐生菌。 Vac突变率（1%）是100倍， 这些特征鲜明的紫膜突变 细菌视蛋白（BOP）插入失活的结果 基因（DasSarma，等人，（未出版），我们现在处于理想状态 位置研究突变，结构，和调节的 使用分子遗传学的标准方法检测GVH基因。 我们计划 （a）研究基因的物理和转录状态， 通过Southern和北方分析， 杂交;和（B）完成gvh基因的DNA序列 区域，并通过S1定位确定mRNA的结构 核酸酶和加帽酶。

The extremely halophilic archaebacterium, Halobacterium halobium, has the capability to synthesize intracellular gas-filled vesicles for buoyancy to regulate the availability of light and oxygen. The mutation rate for gas vesicle synthesis (Vac) is the highest of several phenotypic hypervariabilities observed in H. halobium. The Vac mutation rate (1%) is 100-fold higher than for the well-characterized purple membrane mutations which are the result of insertional inactivation of the bacterio-opsin (bop gene (DasSarma, et al., unpublished), we are now in an ideal position to study the mutation, structure, and regulation of the gvh gene using standard methods of molecular genetics. We plan to: (a) examine the physical and transcriptional state of the gvh gene region in many Vac mutants by Southern and Northern hybridizations; and (b) complete the DNA sequence of the gvh gene region and determine the structure of the mRNA by mapping with S1 nuclease and capping enzyme.