Biophysical Properties of the Bacteriophage T4 Capsid Protein

调节亚细胞成分的大小和形状是 这是所有细胞都面临的一个主要问题。 核糖体，肌肉厚 纤维和中心粒的尺寸相对固定 而其它组分如细胞骨架网络 仅在细胞周期的特定时间固定尺寸。 噬菌体T4甚至头部长度的调节可能是 被认为是系统地探索 这个问题的分子机制，主要是 因为它们的基因非常明确， available. 此外，生物物理学也有很多知识， 主要衣壳的结构和生物化学 蛋白 头部长度调节有两种模式， 游标和动力学模型。 每个机制将 测试以确定主要头部蛋白在 长度测定 为了建立一套 规则定义的类型的氨基酸变化的小 巨大的，ptg和娇小的，pt突变体，调查员将 在另外的PTG位点选择定点突变。 是 可能主要衣壳蛋白gp23中的pt突变 可以增加这种蛋白质的“内在曲率”， 因此产生比正常衣壳短衣壳。 这些实验 希望能得出结论 尝试将 使GP 23结晶。 本组织过去开展的工作 调查员，虽然在不同领域，是优秀的。 林博士当然有能力进行这项工作 并且程序建议优先支持。

Regulation of the size and shape of subcellular components is a major problem faced by all cells. Ribosomes, muscle thick filaments and centrioles have relatively fixed dimensions while other components such as cytoskeletal networks have fixed dimensions only at certain times in the cell cycle. Regulation of the bacteriophage T4 even head length may be considered a prototype for systematically exploring the molecular mechanisms underlying this problem, primarily because of the extremely well defined and versatile genetics available. Furthermore, much is also known of the biophysics of the architecture, and the biochemistry of the major capsid protein. There are two models for head length regulation, the vernier and the kinetic models. Each mechanism will be tested to determine the role of the major head protein in length determination. In an attempt to establish a set of rules defining the types of amino acid changes in the petite giant, ptg and petite, pt mutants, the investigator will select site directed mutations at additional ptg site. It is possible that pt mutations in the major capsid protein gp 23 may increase the "intrinsic curvature" of this protein and hence produce shorter than normal capsids. These experiments hopefully will generate conclusive results. Attempts will be made to crystalize gp 23. The past work conducted by this investigator, although in a different area, was excellent. Dr. Eiserling is certainly capable of carrying out this work and the program recommends support with a high priority.