Imaging mammalian organogenesis with adaptive optics

分析细胞运动的光学和计算障碍限制了我们对哺乳动物器官发生的理解。我们已经建立了一个具有自适应光学的超分辨率旋转盘共聚焦显微镜，并开发了基于机器学习的图像处理和细胞分割工作流程，以克服这些长期存在的障碍。我们建议将这些前沿的实时成像和分析方法结合起来，以表征细胞运动在哺乳动物器官形成中的作用，并开发先进的细胞分割和跟踪方法，用于科学界。我们预计这个项目将对细胞如何相互作用来构建复杂器官产生根本性的见解。

Optical and computational barriers to analysing cell movement have limited our understanding of mammalian organogenesis. We have built a super-resolution spinning disk confocal microscope with adaptive optics and developed machine learning-based image processing and cell segmentation workflows to overcome these long-standing barriers. We propose to combine these cutting-edge live imaging and analysis approaches to characterise the role of cell movement in mammalian organ formation and develop advanced cell segmentation and tracking methods for use in the scientific community. We anticipate this project will generate fundamental insights into how cells interact to build complex organs.