Fundamental Studies of Polymeric Biphasic Systems for Bioseparations

生物源性物质的生产， 基因工程方法依赖于有效的分离 和纯化技术。 该提案旨在使 基于生物分子分配的纯化方法 在含水体系中的两个或更多个不混溶相之间。 的 这项建议的具体目标是： - 使用聚合物表征水性两相体系 溶液热力学 - 使用属性预测分配系数 蛋白质在表面。 这些研究的成功将加强我们对 双水相的分子机理和相互作用 系统. 此外，它还将提供工程原理， 选择合适的系统进行有效分离， 分析和商业规模的操作。 PI被认为具有良好的资质，可以执行 我建议进行研究，并为这项建议提供资金。

The manufacturing of substances of biological origin using genetic engineering methodology depends on efficient separation and purification techniques. This proposal seeks to systematize a purification methodology based on a biomolecular partition between two or more immiscible phases in an aqueous system. The specific objectives of this proposal are to: - Characterize aqueous biphasic systems using polymer solution thermodynamics. - Predict partition coefficients using the properties of proteins at surfaces. The success of these studies will strengthen our understanding of the molecular mechanisms and interactions of aqueous two-phase systems. In addition, it will provide engineering principles for selecting the proper system for effective separations at analytical and commercial scale operations. The PI is considered to be well qualified to carry out the proposed research and I recommend funding of this proposal.