Immunogenetic Studies of a Marsupial (Monodelphis Domestica)

在未来一年，这项研究将集中完成在以前的支持下开始的几个项目。本文将采用Western blot方法对有袋动物家养单足动物主要组织相容性复合体的I类膜抗原进行分离和鉴定。将完成对基线免疫反应的研究，并将继续尝试使新生儿耐受颗粒性和可溶性抗原。关于淋巴细胞对有丝分裂原的反应和单淋巴细胞在混合淋巴细胞培养中的行为的进一步数据将得到。今后将继续研究甲胎蛋白在不同阶段动物体内的发育情况，并研制出抗甲胎蛋白的血清。最后，将继续研究已经确定的酶多态性和血型的遗传控制。本项目主要目的是研究有袋动物的免疫遗传和生化遗传系统。另一个目标是让聪明的本科生在一个成熟而活跃的研究实验室里参与基础研究。

In the coming year, this research will concentrate on completing several of the projects begun under previous support. The Class I membrane antigens of the Major Histocompatibility Complex of the marsupial Monodelphis domestica will be isolated and characterized using the Western blot procedure. Studies of the baseline immune responses will be completed and attempts to tolerize newborns to particulate and soluble antigens will be continued. Further data on the response of lymphocytes to mitogens and the behavior of Monodelphis lymphocytes in mixed lymphocyte cultures will be obtained. Studies will be continued of the development of alpha- fetoprotein in animals of various stages, and antisera against alpha- fetoprotein will be produced. Finally, studies will be continued of the genetic control of the enzyme polymorphisms and blood groups already defined. The major objective of this project is to study the immunogenetic and biochemical genetic systems of a marsupial. Another objective is to involve bright undergraduate students in basic research in an established and active research laboratory.