Discovery and directed evolution of small molecule biosensors

该项目旨在解决不同领域对新型小分子生物传感能力的需求，包括食品和葡萄酒生产、环境监测、生物催化和使用合成生物学方法进行诊断。这项工作的意义是由一个强大的跨学科团队开发新的生物传感器，贡献生物信息学来识别新的生物传感器、创新的蛋白质工程方法和尖端的定向进化方法。预期的成果包括加强跨学科合作的机构能力；发现根本重要的细菌传感器；以及开发能够促进非生物生物催化行业发展的合成监管电路。

This project aims to address the need for novel small molecule biosensing capability in diverse fields including food and wine production, environmental monitoring, biocatalysis, and diagnostics using a synthetic biology approach. The significance of this work is the development of new biosensors by a strong interdisciplinary team contributing bioinformatics to identify new biosensors, innovative protein engineering approaches, and cutting-edge directed evolution methodologies. Intended outcomes include enhanced institutional capacity for interdisciplinary collaboration; discovery of fundamentally important bacterial sensors; and development of synthetic regulatory circuits enabling outgrowth of non-biological biocatalysis industries.