Discovery and directed evolution of small molecule biosensors

该项目旨在利用合成生物学方法解决在食品和葡萄酒生产、环境监测、生物催化和诊断等不同领域对新型小分子生物传感能力的需求。这项工作的意义在于由一个强大的跨学科团队开发新的生物传感器，贡献生物信息学来识别新的生物传感器，创新的蛋白质工程方法和前沿的定向进化方法。预期成果包括加强跨学科合作的机构能力；发现至关重要的细菌传感器；以及合成调控电路的发展，使非生物生物催化工业得以发展。

This project aims to address the need for novel small molecule biosensing capability in diverse fields including food and wine production, environmental monitoring, biocatalysis, and diagnostics using a synthetic biology approach. The significance of this work is the development of new biosensors by a strong interdisciplinary team contributing bioinformatics to identify new biosensors, innovative protein engineering approaches, and cutting-edge directed evolution methodologies. Intended outcomes include enhanced institutional capacity for interdisciplinary collaboration; discovery of fundamentally important bacterial sensors; and development of synthetic regulatory circuits enabling outgrowth of non-biological biocatalysis industries.