Genome Structure and Replication of a Plant Rhabdovirus

本研究有三个主要目标：（1）继续 苦苣苔属植物核糖核酸的结构与组织研究 黄网病毒（SYNV），一种植物弹状病毒; 2）感染 烟草原生质体与SYNV，使动力学和亚细胞 病毒特异性RNA和蛋白质的位置可以是 在同步复制期间进行调查;以及3）隔离 一种来自受感染原生质体的转录酶 完成 目的l将提供一个精确的基因组描述， 一种植物弹状病毒，可以用来比较它的结构， 功能和与相关动物弹状病毒的进化。 用SYNV感染原生质体（目标2）将允许 SYNV RNA合成的动力学分析， 蛋白质的复制阶段，并将允许 研究它们与宿主多肽结合， 在感染过程中出现的细胞病变结构。 隔离 转录酶复合物（目标3）从感染 原生质体将有助于在体外研究的功能 病毒特异性成分和对宿主的要求 信使RNA转录和复制过程中的因子 SYNV RNA的

This study has three primary objectives: l) to continue studies on the structure and organization of the RNA of sonchus yellow net virus (SYNV), a plant rhabdovirus; 2) to infect tobacco protoplasts with SYNV so that kinetics and subcellular location of the viral specific RNAs and proteins can be investigated during synchronous replication; and 3) to isolate a transcriptase from infected protoplasts. Completion of objective l will provide a precise description of the genome of a plant rhabdovirus that can be used to compare its structure, function, and evolution with related animal rhabdoviruses. Infection of protoplasts with SYNV (Objective 2) will allow analysis of the kinetics of synthesis of SYNV RNAs and proteins at defined stages of replication and will permit investigation of their association with host polypeptides and cytopathic structures that appear during infection. Isolation of a transcriptase complex (Objective 3) from infected protoplasts will facilitate in vitro studies of the functions of viral specific components and the requirement for host factors during transcription of messenger RNAs and replication of SYNV RNA.