Virus Expression in Plants

花椰菜花叶病毒(CaMV)是一种含有植物病毒的双链DNA，它对病害症状的表达进行了研究。这项工作将集中在一种特定的病毒基因CaMV基因VI的调控上，该基因的蛋白产物与一种疾病表型的产生有关。将提出的问题包括确定基因VI的产物是否调节其自身的表达，如果是的话，确定涉及蛋白质的哪些区域。这些研究将在转基因植物和瞬时表达系统中进行。基因VI启动子活性的定量将使用荧光素酶报告基因系统来获得。

A study will be made of the expression of disease symptons by Cauliflower Mosaic Virus (CaMV), a double strand DNA containing plant virus. This work will focus on the regulation of a particular viral gene, CaMV gene VI, whose protein product has been implicated in production of a disease phenotype. Questions that will be asked include determining whether the product of gene VI regulates its own expression and, if so, determining which domains of the protein are involved. These studies will be carried out in both transgenic plants and in transient expression systems. Quantitation of gene VI promoter activity will be obtained by use of the luciferase reporter gene system.