A Study of Tissue-, Ontogenetic- and Environmental Specificity of Gibberellin Biosynthesis Mutants in Pisum Sativum

本研究旨在研究豌豆赤霉素生物合成突变体。LE和NA突变等位基因阻止了赤霉素的生物合成，每个等位基因都位于赤霉素途径的特定位置。提出了用毛细管气相色谱-质谱法对等基因系组成器官中赤霉素含量进行定量的方法。通过监测赤霉素水平，将有可能确定这些基因的组织特异性和个体发育特异性。这项研究的意义在于有可能确定内源赤霉素A1的水平如何导致矮豌豆的表型。赤霉素A1是调节节间伸长的活跃因子。这项工作产生的发现将为未来更多的分子研究奠定基础，它们可能会导致发现有商业价值的豌豆，即具有正常豆荚大小的矮豌豆。

The purpose of the proposed research is to investigate two gibberellin biosynthesis mutants of Pisum sativum. The le and na mutant alleles block the biosynthesis of gibberellins, each at a specific site in the gibberellin pathway. It is proposed to quantify gibberellin levels in constituent organs of the isogenic lines througout development, using capillary gas chromatography- mass spectrometry. By monitoring gibberellin levels it will be possible to determine the tissue-specificity and ontogenetic specificity of these genes. The significance of this research is the potential to determine how the levels of the endogenous gibberellin A1, which is active in regulating internode extension, causes a dwarf pea phenotype. The findings generated by this work will lay the foundation for more molecular studies in the future and they may lead to the discovery of commercially useful peas, i.e., dwarf pea with normal pod size.