Clonal Variation and Metabolism in Enteric Bacteria

这项研究旨在阐明遗传， 基因克隆变异的分子生物学基础 表达及其与代谢的关系 细菌 细菌碱性磷酸酶的克隆变异 将在> phoR>突变体中进行检查，其中这种新形式的 观察基因调控。 遗传基础将被检查 通过鉴定转座子诱导的突变， 变化量 代表性的突变将被分子克隆， 绘制并通过侧翼的直接DNA测序进行分析 DNA 目前的目标将是确定基因， 这种变化使得我们可以制作模型来解释 变异的分子和生化基础。这样的模型 这将是继续这项研究的基础。

This study is directed towards elucidating the genetic, molecular, and biochemical basis of clonal variation of gene expression and its relationship to metabolism in enteric bacteria. Clonal variation of bacterial alkaline phosphatase will be examined in >phoR> mutants, in which this novel form of gene regulation is observed. The genetic basis will be examined by identifying transposon.induced mutations that alter clonal variation. Representative mutations will be molecularly cloned, mapped, and analyzed by direct DNA sequencing of the flanking DNA's. The immediate goal will be to identify genes that affect the variation to allow making models that could explain the molecular and biochemical basis of the variation. Such models will form the basis for continuing this study.