U.S.-Korea Cooperative Research: Reduction of NAD+ Analoguesby Phosphonate

该提案要求提供资金，以允许莫里斯·M。克里沃伊 明尼苏达大学化学系， In-Sook Han Lee博士，化学系，科学教育 Kangweon国立大学研究所，为期24年 + 几个月来，一项关于减少NAD的合作研究计划 类似物（烟酰胺季铵盐），包括吡啶， 喹啉鎓、吖啶鎓和菲啶鎓卤化物，通过单或 磷酸二钠 将探讨这种减少的性质， 通过仪器技术和 通过动力学方法确定反应机理。 还原烟酰胺季盐及其类似物， 这是一个长期的目标，因为这一点非常重要。 生物系统中的方法和烟酰胺的可能用途 合成转化中的酶。 尽管这些反应 在实际的有机合成中可能有价值，酶催化 这种类型的氧化还原反应还不是很 成功是因为减少的辅因子生产昂贵，一些 不是市售的，有些在溶液中不稳定， 其它留下不希望的副产物。 膦酸盐用作还原剂 代理商有几个优点，即它是一个足够强大的还原 + 一种能与多种NAD产生广泛反应的试剂 类似物，并且它是廉价的、可商购的、稳定的， 溶液，其最终产物不太可能产生副反应。 该项目与美国的目标有关-韩国 合作科学计划，旨在提高 美国和韩国科学家和工程师之间的合作， 科学信息、思想、技能和技术的交流 并通过在互利问题上的合作。 这个项目 将为克里沃伊博士的 在NSF批准号CHE-8520903下进行的研究。

This proposal requests funds to permit Dr. Maurice M. Kreevoy, Department of Chemistry, University of Minnesota, to pursue with Dr. In-Sook Han Lee, Department of Chemistry, Science Education Research Institute, Kangweon National University, for a period of 24 + months, a program of cooperative research on the reduction of NAD analogues (nicotinamide quarternary salts), including pyridinium, quinolinium, acridinium, and phenanthridinium halides, by mono- or disodium phosphonate. The nature of this reduction will be explored by characterizing the products by instrumental techniques and by determining the mechanism of the reaction by kinetic methods. The reduction of nicotinamide quarternary salts and their analogs has been a long standing objective because of the great importance of this process in biological systems and the possible use of nicotinamide enzymes in synthetic transformation. Although many of these reactions could be valuable in practical organic synthesis, enzyme-catalyzed oxidation-reduction reactions of this type have not been very successful because the reduced cofactors are expensive to produce, some are not commercially available, some are unstable in solution, and others leave undesirable by-products. Phosphonate used as a reducing agent has several advantages, namely, it is a strong enough reducing + agent to produce a wide range of reactions with a variety of NAD analogs and it is inexpensive, commercially available, stable in solution, and its end product is not likely to produce side reactions. This project is relevant to the objectives of the U.S.-Korea Cooperative Science Program which seeks to increase the level of cooperation between U.S. and Korean scientists and engineers through the exchange of scientific information, ideas, skills, and techniques and through collaboration on problems of mutual benefit. This project will add an international cooperative dimension to Dr. Kreevoy's research under NSF Grant No. CHE-8520903.